Table of Contents
Chromatography Research International
Volume 2012, Article ID 713273, 8 pages
Research Article

Analysis of Some Biogenic Amines by Micellar Liquid Chromatography

Chair of Physical Chemistry, Department of Planar Chromatography, Faculty of Chemistry, Maria Curie Skłodowska University, M. Curie—Skłodowska Sq. 3, 20-031 Lublin, Poland

Received 10 February 2012; Revised 8 May 2012; Accepted 22 May 2012

Academic Editor: Burcu Dogan-Topal

Copyright © 2012 Irena Malinowska and Katarzyna E. Stępnik. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Micellar liquid chromatography (MLC) with the use of high performance liquid chromatography (HPLC) was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine. In this paper, an influence of surfactant’s concentration and pH of the micellar mobile phase on the retention of the tested substances was examined. To determine the influence of surfactant’s concentration on the retention of the tested amines, buffered solutions (at pH 7.4) of ionic surfactant—sodium dodecyl sulfate SDS (at different concentrations) with acetonitrile as an organic modifier (0.8/0.2 v/v) were used as the micellar mobile phases. To determine the influence of pH of the micellar mobile phase on the retention, mobile phases contained buffered solutions (at different pH values) of sodium dodecyl sulfate SDS (at 0.1 M) with acetonitrile (0.8/0.2 v/v). The inverse of value of retention factor ( 1 / π‘˜ ) versus concentration of micelles ( 𝐢 𝑀 ) relationships were examined. Other physicochemical parameters of solutes such as an association constant analyte—micelle ( 𝐾 m a )—and partition coefficient of analyte between stationary phase and water (hydrophobicity descriptor) ( 𝑃 s w Ξ¦ ) were determined by the use of Foley’s equation.