Research Article
Development and Validation of a Stability Indicating LC Method for the Assay and Related Substances Determination of a Proteasome Inhibitor Bortezomib
| (a) |
| | Trial Number | Mobile phase A | Composition ratio of mobile phase A |
| | 1 | Acetonitrile : Water : Formic acid | 500 : 500 : 1 | | 2 | Acetonitrile : Water : Formic acid | 300 : 700 : 1 | | 3 | Acetonitrile : Water : Formic acid | 250 : 750 : 1 | | 4 | Acetonitrile : Water : Formic acid | 285 : 715 : 1 | | 5 | Acetonitrile : Water : Formic acid | 285 : 715 : 1 |
|
|
Mobile phase A used for different trials.
|
| (b) |
| | Trial Number | Mobile phase B | Composition ratio of mobile phase B |
| | 1 | Acetonitrile : Water : Formic acid | 500 : 500 : 1 | | 2 | Acetonitrile : Water : Formic acid | 800 : 200 : 1 | | 3 | Acetonitrile : Water : Formic acid | 800 : 200 : 1 | | 4 | Acetonitrile : Water : Formic acid | 800 : 200 : 1 | | 5 | Methanol : Water : Formic acid | 800 : 200 : 1 |
|
|
Mobile phase B used for different trials.
|
