Table of Contents
Chromatography Research International
Volume 2012, Article ID 812127, 8 pages
Research Article

Separation of Polyphenols from Jordanian Olive Oil Mill Wastewater

Department of Chemistry, University of Jordan, P.O. Box 13003, Amman 11942, Jordan

Received 18 February 2012; Accepted 10 April 2012

Academic Editor: Eva Pocurull

Copyright © 2012 Ahmad A. Deeb et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This research aims at separation of polyphenols from Jordanian olive mill wastewater which have possible applications in pharmaceutical industry. The phenolic compounds were isolated using silica column chromatography based on using different solvents after extracting the acidified solution with n-hexane and ethyl acetate. The structural elucidation of the separated compounds was achieved using 1H-NMR, 13C-NMR and mass spectrometry. The concentrations of these compounds were determined by GC-MS after derivatization with N, O-bis(trimethylsilyl)trifluoroacetamide (BSTFA). The concentrations of the main isolated phenolic compounds in the Jordanian olive mill wastewater were ferulic acid (93.6 mg/L), trans-cinnamic acid (105.3 mg/L), p-coumaric acid (117.0 mg/L), vanillic acid (128.7 mg/L), caffeic acid (140.4 mg/L), tyrosol (210.6 mg/L), and hydroxytyrosol (315.9 mg/L).