Table of Contents
Chromatography Research International
Volume 2013, Article ID 315145, 12 pages
Research Article

Development and Validation of a Stability-Indicating RP-HPLC Method for the Simultaneous Estimation of Guaifenesin and Dextromethorphan Impurities in Pharmaceutical Formulations

1Analytical Research and Development, Integrated Product Development, Dr. Reddy’s Laboratories Limited, Bachupally, Hyderabad-500072, India
2School of Chemistry, Andhra University, Visakhapatnam, Andhra Pradesh 530003, India

Received 8 June 2013; Revised 9 August 2013; Accepted 9 August 2013

Academic Editor: Osama Y. Aldirbashi

Copyright © 2013 Thummala V. Raghava Raju et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Accuracy, precision at lower and higher levels,

Accuracy of the method was evaluated by using concentration levels LOQ, 0.1%, 0.2%, 0.4%, 0.8% and 1.0% on GN + DN tablets. Six preparations were performed at LOQ & 1.0% level and three preparations were performed at different levels. The recovery of all the impurities from finished pharmaceutical dosage form ranged from 85.0 % to 115.0 %. The summary of % recovery for individual impurity was mentioned in Table 1 to Table 6.

From Figure 1a and 1b (Different scale chromatograms, for better clarity), it was clearly shows that all the known impurities were well separated from main peaks and placebo peaks.

  1. Supplementary Material