Table of Contents
Chromatography Research International
Volume 2013, Article ID 509812, 7 pages
http://dx.doi.org/10.1155/2013/509812
Review Article

Recycle HPLC: A Powerful Tool for the Purification of Natural Products

School of Pharmaceutical Sciences, Faculty of Applied Medical Sciences, Lovely Professional University, Punjab-144411, India

Received 5 March 2013; Revised 17 September 2013; Accepted 2 October 2013

Academic Editor: Andrew Shalliker

Copyright © 2013 Jasmeen Sidana and Lokesh Kumar Joshi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Natural compounds occur as various isomeric or closely related structures in biological matrices. These compounds are difficult to separate from the complex mixtures, and hence, the need for effective and innovative separation techniques arises. Recycle HPLC allows the recycling of sample, in part or full, and increases the separation efficiency of the process while keeping the peak dispersion to a minimum. Recycling in an HPLC system has been used in the isolation and purification of different types of natural products including enantiomers, diastereomers, epimers, positional isomers, and structurally related or unrelated compounds having similar retention characteristics. The present paper overviews the development of instrumentation and setup of recycle HPLC and its applications in the separation of natural products.