Table of Contents
Chromatography Research International
Volume 2013, Article ID 673150, 5 pages
http://dx.doi.org/10.1155/2013/673150
Research Article

A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study

1Department of Chemistry, Saurashtra University, Rajkot, Gujarat 360005, India
2Banasthali Vidyapith, P.O. BanasthaliVidyapith, Rajasthan 304022, India

Received 18 September 2013; Accepted 27 October 2013

Academic Editor: Toyohide Takeuchi

Copyright © 2013 Hetal Jebaliya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The simplest stability indicating reversed phase Isocratic HPLC and UPLC methods has been developed and validated for the determination of fluconazole in bulk and solid pharmaceutical dosage form. A SunFire C18 (250 × 4.5 mm, 5 μm particle size) column has been used for HPLC and BEH C18 (100 × 2.1 mm, 1.7 μm particle size) column used for UPLC. The Mobile phase consisted of Methanol : Water (70 : 30) for HPLC and Methanol : Water (55 : 45 v/v) for UPLC. Isocratic flow was set at 1 mL/min and 0.30 mL/min, respectively, for HPLC and UPLC. For both HPLC and UPLC system detection has been performed at 211 nm with 30°C column oven temperature (good elution was obtained at 30°C) and injection volume, respectively, 2 μL and 20 μL for HPLC and UPLC.