A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study

Table 3

Robustness study data obtained from UPLC analysis.

Robustness conditions		RT (min)	ASSAY (%)	System suitability data
Type	Rate			Theoretical plate	Asymmetry
Flow rate	0.28 mL/min.	1.25	99.85	2792	1.06
	0.32 mL/min.	1.06	101.99	2460	0.71
Temperature	28	1.05	98.95	2361	0.97
	32	1.24	99.55	3194	1.67
Mobile phase	53 : 47	1.26	98.60	2984	1.05
	57 : 43	1.11	99.51	2789	1.10