Chromatography Research International / 2013 / Article / Tab 4

Research Article

A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study

Table 4

Comparison of HPLC and UPLC methods by validation results.

Validation parameterHPLC methodUPLC method

Accuracy (% Recovery)99-10099-100
 Regression equation
 Corelation coefficient ( )0.9840.998
 Intermediate precision(% RSD) 0.61(% RSD) 1.18
 Method precision(% RSD) 0.71(% RSD) 0.70
RobustnessHighly robustness methodHighly robustness method
SpecificityNo interference found to analyte peakNo interference found to analyte peak
Limit of detection0.5 µg/mL0.3 µg/mL
Limit of quantification1.5 µg/mL1.4 µg/mL
Total analysis time7 minutes3 minutes

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