Table of Contents
Chromatography Research International
Volume 2014, Article ID 108694, 5 pages
http://dx.doi.org/10.1155/2014/108694
Research Article

Development and Validation of an HPLC Method for the Analysis of Chlorpropham and 3-Chloroaniline in Potato Extracts

1School of Chemistry, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq
2School of Chemistry, Faculty of Science and Engineering, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK

Received 28 September 2014; Accepted 15 December 2014; Published 29 December 2014

Academic Editor: Toyohide Takeuchi

Copyright © 2014 Nidhal M. Sher Mohammed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chlorpropham (CIPC) is the main sprout inhibitor used by potato industry. There is concern about the residues of CIPC and its degradation product 3-chloroaniline, 3-CA; hence, analytical methods are required to analyse their residues in potato samples. An HPLC-UV method was developed and validated for the separation and quantification of these compounds using propham (IPC) as an internal standard. The chromatographic conditions required to achieve good separation were 60% mobile phase of methanol, 15-minute run time at a flow rate of 1.5 mL/min, and a detection wavelength of 210 nm using Phenomenex (ODS-2 250 mm 4.60 mm 5 µm Sphereclone) column at an ambient temperature. The method was validated for precision, linearity, the limit of detection (LOD) and the limit of quantification (LOQ), producing high precision through RSD ≤ 0.03%, and acceptable criteria of the coefficient of determination () of the calibration curves (0.990). LOD values of CIPC and 3-CA were approximately 0.01 µg/mL whereas the LOQ values were approximately 0.04 µg/mL using repeated injection approach. The proposed HPLC method was compared with the standard GC method of the CIPC residues extracted showing good agreement . Despite using the same extract, the recovery results for the proposed HPLC method were 13% higher than GC analysis.