Extraction of Artemisinin, an Active Antimalarial Phytopharmaceutical from Dried Leaves of Artemisia annua L., Using Microwaves and a Validated HPTLC-Visible Method for Its Quantitative Determination
Table 1
Screening of different mobile phases for optimization of better chromatographic separation between artemisinin and related impurities.
Mobile phase single or binary solvent mixtures (v/v)
Time to travel to solvent front (seconds)
Flow or velocity constant (mm2 s−1)
Thin-layer chromatographic data
Thin-layer plate data
Densitogram data
Specificity of separation (spot spectrum purity, i.e., peak purity data)
Number of theoretical plates ()
Number of theoretical plates ()
Correlation values ()
Up slopea
Down slopeb
-Hexane : diethyl ether, 50 : 50
604.24
11.1280
0.28
3551.11
0.28
766.13
0.999053
0.998898
Methylene dichloride (100%)
755.37
8.9015
0.19
2493.33
0.19
729.96
0.998846
0.997223
-Hexane : ethyl acetate, 75 : 25
699.57
9.6116
0.28
3551.11
0.27
807.54
0.999333
0.999111
-Hexane : acetone, 80 : 20
655.39
10.2595
0.28
3551.11
0.28
770.72
0.999075
0.985880
Chloroform : methanol, 95 : 5
960.22
7.0025
0.60
5662.04
0.60
980.15
0.999280
0.993213
Spot start to spot middle; bspot middle to spot end.