Chromatography Research International / 2014 / Article / Tab 2

Research Article

Validated HPLC Method for Quantification of Pregabalin in Human Plasma Using 1-Fluoro-2,4-dinitrobenzene as Derivatization Agent

Table 2

Precision and accuracy of method for determination of pregabalin in standard solutions (; five sets for 3 days).

Nominal concentration (ng/mL)Intraday ()Interday ()
Determined (ng/mL)RSD (%)Error (%)Determined (ng/mL)RSD (%)Error (%)

1.001.0710.57.01.0812.78.0
5.005.178.13.45.2310.74.6
75.0078.106.24.170.3310.1
450.00438.404.3413.874.7
550.00528.506.4511.178.3
2500.002419.538.62404.678.9
4500.004679.7710.24.04325.1010.4

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