Table of Contents
Chromatography Research International
Volume 2015 (2015), Article ID 757649, 8 pages
http://dx.doi.org/10.1155/2015/757649
Research Article

A Simple Analytical Method for High-Throughput Screening of Major Sugars from Soybean by Normal-Phase HPLC with Evaporative Light Scattering Detection

National Center for Soybean Biotechnology and Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA

Received 26 March 2015; Accepted 16 June 2015

Academic Editor: Masami Shibukawa

Copyright © 2015 Babu Valliyodan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. J. Rackis, “Oligosaccharides of food legumes: alphagalactosidase activity and flatulence problem,” in Physilogical Effects of Food Carbohydrates, A. Jeanes and J. Hodge, Eds., pp. 207–222, American Chemical Society, Washington, DC, USA, 1975. View at Google Scholar
  2. F. L. Suarez, J. Springfield, J. K. Furne, T. T. Lohrmann, P. S. Kerr, and M. D. Levitt, “Gas production in humans ingesting a soybean flour derived from beans naturally low in oligosaccharides,” American Journal of Clinical Nutrition, vol. 69, no. 1, pp. 135–139, 1999. View at Google Scholar · View at Scopus
  3. J. D. Neus, W. R. Fehr, and S. R. Schnebly, “Agronomic and seed characteristics of soybean with reduced raffinose and stachyose,” Crop Science, vol. 45, no. 2, pp. 589–592, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. V. Kumar, A. Rani, L. Goyal et al., “Sucrose and raffinose family oligosaccharides (RFOs) in soybean seeds as influenced by genotype and growing location,” Journal of Agricultural and Food Chemistry, vol. 58, no. 8, pp. 5081–5085, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. K. B. Hagely, D. Palmquist, and K. D. Bilyeu, “Classification of distinct seed carbohydrate profiles in soybean,” Journal of Agricultural and Food Chemistry, vol. 61, no. 5, pp. 1105–1111, 2013. View at Publisher · View at Google Scholar · View at Scopus
  6. H. F. Walton, “Ligand-exchange chromatography: a brief review,” Industrial and Engineering Chemistry Research, vol. 34, no. 8, pp. 2553–2554, 1995. View at Publisher · View at Google Scholar · View at Scopus
  7. C. M. Montera, M. C. R. Dodero, D. A. G. Sánchez, and C. G. Barroso, “Analysis of low molecular weight carbohydrates in food and beverages: a review,” Chromatographia, vol. 59, no. 1-2, pp. 15–30, 2004. View at Google Scholar · View at Scopus
  8. L. T. Black and E. B. Bagley, “Determination of oligosaccharides in soybeans by high pressure liquid chromatography using an internal standard,” Journal of the American Oil Chemists' Society, vol. 55, no. 2, pp. 228–232, 1978. View at Publisher · View at Google Scholar · View at Scopus
  9. J. L. Chávez-Servín, A. I. Castellote, and M. C. López-Sabater, “Analysis of mono- and disaccharides in milk-based formulae by high-performance liquid chromatography with refractive index detection,” Journal of Chromatography A, vol. 1043, no. 2, pp. 211–215, 2004. View at Publisher · View at Google Scholar · View at Scopus
  10. J. C. M. Barreira, J. A. Pereira, M. B. P. P. Oliveira, and I. C. F. R. Ferreira, “Sugars profiles of different chestnut (Castanea sativa Mill.) and almond (Prunus dulcis) cultivars by HPLC-RI,” Plant Foods for Human Nutrition, vol. 65, no. 1, pp. 38–43, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. F. N. Lamari, R. Kuhn, and N. K. Karamanos, “Derivatization of carbohydrates for chromatographic, electrophoretic and mass spectrometric structure analysis,” Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 793, no. 1, pp. 15–36, 2003. View at Publisher · View at Google Scholar · View at Scopus
  12. H. K. Joung, H. Huang, W. K. Gyoung, and J. C. Won, “Simultaneous quantitative determination of monosaccharides including fructose in hydrolysates of yogurt and orange juice products by derivatization of monosaccharides with p-aminobenzoic acid ethyl ester followed by HPLC,” Bulletin of the Korean Chemical Society, vol. 26, no. 10, pp. 1533–1538, 2005. View at Publisher · View at Google Scholar · View at Scopus
  13. R. Masuda, K. Kaneko, and I. Yamashita, “Sugar and cyclitol determination in vegetables by HPLC using postcolumn fluorescent derivatization,” Journal of Food Science, vol. 61, no. 6, pp. 1186–1190, 1996. View at Publisher · View at Google Scholar · View at Scopus
  14. A. Hou, P. Chen, A. Shi, B. Zhang, and Y.-J. Wang, “Sugar variation in soybean seed assessed with a rapid extraction and quantification method,” International Journal of Agronomy, vol. 2009, Article ID 484571, 8 pages, 2009. View at Publisher · View at Google Scholar
  15. S. Ouchemoukh, P. Schweitzer, M. B. Bey, H. Djoudad-Kadji, and H. Louaileche, “HPLC sugar profiles of Algerian honeys,” Food Chemistry, vol. 121, no. 2, pp. 561–568, 2010. View at Publisher · View at Google Scholar · View at Scopus
  16. W. Widmer, “Analysis of biomass sugars and galacturonic acid by gradient anion exchange chromatography and pulsed amperometric detection without post-column addition,” Biotechnology Letters, vol. 33, no. 2, pp. 365–368, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. C. Corradini, A. Cavazza, and C. Bignardi, “High-performance anion-exchange chromatography coupled with pulsed electrochemical detection as a powerful tool to evaluate carbohydrates of food interest: principles and applications,” International Journal of Carbohydrate Chemistry, vol. 2012, Article ID 487564, 13 pages, 2012. View at Publisher · View at Google Scholar
  18. C. Ma, Z. Sun, C. Chen, L. Zhang, and S. Zhu, “Simultaneous separation and determination of fructose, sorbitol, glucose and sucrose in fruits by HPLC-ELSD,” Food Chemistry, vol. 145, pp. 784–788, 2014. View at Publisher · View at Google Scholar · View at Scopus
  19. P. Shanmugavelan, S. Y. Kim, J. B. Kim et al., “Evaluation of sugar content and composition in commonly consumed Korean vegetables, fruits, cereals, seed plants, and leaves by HPLC-ELSD,” Carbohydrate Research, vol. 380, pp. 112–117, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. X. Liu, N. Ai, H. Zhang et al., “Quantification of glucose, xylose, arabinose, furfural, and HMF in corncob hydrolysate by HPLC-PDA-ELSD,” Carbohydrate Research, vol. 353, pp. 111–114, 2012. View at Publisher · View at Google Scholar · View at Scopus
  21. U. Sharma, P. Bhandari, N. Kumar, and B. Singh, “Simultaneous determination of ten sugars in Tinospora cordifolia by ultrasonic assisted extraction and LC-ELSD,” Chromatographia, vol. 71, no. 7-8, pp. 633–638, 2010. View at Publisher · View at Google Scholar · View at Scopus
  22. F. A. Agblevor, B. R. Hames, D. Schell, and H. L. Chum, “Analysis of biomass sugars using a novel HPLC method,” Applied Biochemistry and Biotechnology, vol. 136, no. 3, pp. 309–326, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. R. Slimestad and I. M. Vågen, “Thermal stability of glucose and other sugar aldoses in normal phase high performance liquid chromatography,” Journal of Chromatography A, vol. 1118, no. 2, pp. 281–284, 2006. View at Publisher · View at Google Scholar · View at Scopus
  24. K. Downes and L. A. Terry, “A new acetonitrile-free mobile phase method for LC-ELSD quantification of fructooligosaccharides in onion (Allium cepa L.),” Talanta, vol. 82, no. 1, pp. 118–124, 2010. View at Publisher · View at Google Scholar · View at Scopus
  25. R. Slimestad, R. Seljaasen, K. Meijer, and S. L. Skar, “Norwegian-grown Jerusalem artichoke (Helianthus tuberosus L.): morphology and content of sugars and fructo-oligosaccharides in stems and tubers,” Journal of the Science of Food and Agriculture, vol. 90, no. 6, pp. 956–964, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. E. M. Bainy, S. M. Tosh, M. Corredig, V. Poysa, and L. Woodrow, “Varietal differences of carbohydrates in defatted soybean flour and soy protein isolate by-products,” Carbohydrate Polymers, vol. 72, no. 4, pp. 664–672, 2008. View at Publisher · View at Google Scholar · View at Scopus
  27. T. M. Kuo, J. F. vanMiddlesworth, and W. J. Wolf, “Content of raffinose oligosaccharides and sucrose in various plant seeds,” Journal of Agricultural and Food Chemistry, vol. 36, no. 1, pp. 32–36, 1988. View at Publisher · View at Google Scholar · View at Scopus
  28. E. Giannoccaro, Y.-J. Wang, and P. Chen, “Effects of solvent, temperature, time, solvent-to-sample ratio, sample size, and defatting on the extraction of soluble sugars in soybean,” Journal of Food Science, vol. 71, no. 1, pp. C59–C64, 2006. View at Publisher · View at Google Scholar · View at Scopus
  29. Y. Lv, X. B. Yang, Y. Zhao, Y. Ruan, Y. Yang, and Z. Z. Wang, “Separation and quantification of component monosaccharides of the tea polysaccharides from Gynostemma pentaphyllum by HPLC with indirect UV detection,” Food Chemistry, vol. 112, no. 3, pp. 742–746, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. A. Hou, P. Chen, J. Alloatti et al., “Genetic variability of seed sugar content in worldwide soybean germplasm collections,” Crop Science, vol. 49, no. 3, pp. 903–912, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. L. Fang, M. Wan, M. Pennacchio, and J. Pan, “Evaluation of evaporative light-scattering detector for combinatorial library quantitation by reversed phase HPLC,” Journal of Combinatorial Chemistry, vol. 2, no. 3, pp. 254–257, 2000. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Man, W. Gao, Y. Zhang et al., “Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC-ELSD and HPLC-MS/MS,” Journal of Chromatography B, vol. 878, no. 29, pp. 2943–2948, 2010. View at Publisher · View at Google Scholar · View at Scopus
  33. Y. Tang, W. Jiang, Q. Wu et al., “Comparative characteristic of the inflammatory diterpenes in the roots of Euphorbia fischeriana with different preparation method using HPLC-ELSD,” Fitoterapia, vol. 83, no. 3, pp. 427–433, 2012. View at Publisher · View at Google Scholar · View at Scopus