Validated Stability Indicating Chromatographic Method for the Simultaneous Estimation of Camylofin with NSAID Drugs and a New Approach of Method Transfer from Classical HPLC to a Modern UPLC Instrument

Table 6

Significant values in comparison with UPLC and HPLC.

Sr. no.	Chromatographic conditions	Instrumentation
		UPLC		HPLC
1	Flow (mL/min)	0.250		1.0
2	Injection volume (L)	1.0		20.0
3	Amount loaded on column (g)	0.05		1.00
4	Run time (min)	5.0		20.0
5	Drugs	CMF	DCF	CMF	DCF
6	Tailing factor ()	1.483	0.956	1.33	1.34
7	Resolution ()	11.87		25.90
8	Retention time (min)	1.1	3.2	2.6	13.7
9	Theoretical plates ()	1144	3296	2678	7534

Significance of HPLC to UPLC method transfer.