Development and Validation of RP-HPLC Method for the Determination of Hydrochlorothiazide in Bulk Drug and Pharmaceutical Dosage Form

Table 1

The effect of different mobile phase strengths on the peak area and retention time of HCTZ.

Mobile phase (ACN : H2O)	(min)	Peak area
50 : 50	3.37	384763
60 : 40	3.14	386386
70 : 30	3.01	390123