Development and Validation of New RP-HPLC Method for Simultaneous Determination of Methyl and Propyl Parabens with Levetiracetam in Pure Form and Pharmaceutical Formulation

Table 1

Regression and statistical parameters for determination of LTC, MHB, and PHB using the proposed HPLC method.

Parameter	Levetiracetam	Methyl paraben	Propyl paraben
System suitability
±  SD (min)	1.561 ± 8.22 × 10^−3	5.855 ± 0.6 × 10^−2	7.844 ± 7.8 × 10^−2
USP plate count ()	3.36 × 10^3	3.59 × 10^4	9.04 × 10^4
Resolution (Rs)	—	3.7	4.45
Symmetry factor ()	1.08	1.04	0.996
Selectivity (α)	—	4.91	1.08
Linearity and regression data
Linearity range (µgmL^−1)	500–1500	10–30	1–3
LOD (µgmL^−1)	0.0078	0.025	0.029
LOQ (µgmL^−1)	0.023	0.076	0.088
Slope ()	220896	21738	31501
Intercept ()	3880	−3407	5574.4
Correlation coefficient ()	0.9996	0.9999	0.9994

LOD: limit of detection; LOQ: limit of quantitation.
Regression equation for the ratio of peak area of the analyte against concentration of the analyte.