Upper Gastrointestinal Bleeding from Gastric Amyloidosis in a Patient with Smoldering Multiple Myeloma

Figure 3

Congo red-positive sections viewed under ultraviolet light in the microdissection microscope. The amyloid fluoresces red. The drawn lines (yellow in (a), green in (b), and blue in (c)) delineate areas circumscribing the amyloid deposits that were cut out of the tissue sections for processing for mass spectrometry. The three dissections in (a)–(c) correspond to the three patient sample columns in the Scaffold illustration in Table 1. Each sample was processed in triplicate. (d) shows the amyloid fragments that have been cut out of the tissue and are now in the reaction cup for further processing (i.e., reduction of disulfide bonds, trypsin digestion, etc.) before mass spectrometry.

(a)

(b)

(c)

(d)