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Case Reports in Gastrointestinal Medicine
Volume 2016 (2016), Article ID 8356708, 3 pages
http://dx.doi.org/10.1155/2016/8356708
Case Report

The Role of PCR in Diagnosis of a Rare Appendicular Tuberculosis and Mini Literature Review

1Pathology Department, Faculty of Medicine, University of Gezira, P.O. Box 20, Wad Madani, Al Gezira, Sudan
2Pathology Department, Gezira Medical Laboratory, Wad Madani, Al Gezira, Sudan

Received 24 January 2016; Accepted 17 March 2016

Academic Editor: Olga I. Giouleme

Copyright © 2016 Asmerom Tesfamariam Sengal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tuberculosis is a prevalent public health problem especially in the poor developing countries and results in significant mortality. Albeit tuberculosis almost always affects any organ or system of the body, abdominal tuberculosis is less frequent; moreover, tuberculous appendicitis is very rare with an incidence estimated at about 0.1–0.6% of all gastrointestinal tuberculosis. The purpose of this report was to present an unusual case of primary tuberculous appendicitis and the approach used for accurate diagnosis as well as a current update on the disease. We are reporting a 30-year-old male who presented with acute abdominal pain, fever, and vomiting and was admitted with the clinical diagnosis of acute appendicitis. Patient was investigated thoroughly and histopathologic examination was strongly suggestive of tuberculous appendicitis; however, Ziehl-Neelsen (ZN) was negative in tissue section. To confirm the diagnosis, molecular biology [polymerase chain reaction (PCR)] study was performed from the formalin fixed paraffin embedded (FFPE) appendicular tissue and revealed presence of Mycobacterium tuberculosis. As there are numerous differential diagnoses in granulomatous lesions of appendix and due to the fact that appendicular tuberculosis is a rare phenomenon; verification etiologic agent is crucial for appropriate management of the disease.