Detection of Rare Beta Globin Gene Mutation [+22 5UTR(G>A)] in an Infant, Despite Prenatal Screening

Figure 1

Gel agarose electrophoresis of ARMS-PCR product (522 bp fragment) for the detection of codon 8 (-AA) mutation. Patient sample: lanes 1 and 5, negative (wild type), control: lanes 2 and 6, positive control: lanes 3 and 7,and ladder marker 100 bp: lane 4. Lanes 1, 2, and 3 represent PCR products amplified with primers binding normal allele, and lanes 5, 6, and 7 represent PCR products amplified with primers binding mutant allele.