A Double Hit CD10-Negative B-Cell Lymphoma with t(3;8)(q27;q24) Leading to Juxtaposition of the BCL6 and MYC Loci Associated with Good Clinical Outcome
Morphology, karyotype, and FISH analysis of the case. (a) Blast cells demonstrating basophilic cytoplasm and high nuclear cytoplasm ratio. (b) Bone marrow morphology showing diffuse infiltration. (c) Analysis of bone marrow metaphase using FISH probes. A Vysis LSI MYC dual colour break-apart rearrangement probe was employed to show a MYC fusion on the normal chromosome 8, with the other MYC allele split between the der(3) and der(8). (d) G-banding karyogram showing t(3;8)(q27;q24). ((e), (f), and (g)) Interphase FISH analyses (false colour display derived from the ISIS/MetaSystems FISH system). (e) FISH analysis of the t(3;8) translocation. (e) MYC break-apart probe showing one red/green colocalised signal from the unrearranged locus and two separate red and green signals from the rearranged locus. (f) BCL6 break-apart probe showing one red/green colocalised signal from the unrearranged locus and two separate red and green signals. (g) IGH break-apart probe showing a signal of two colocalised red/green signals indicating no breaks at the IGH locus. (h) MYC/IGH/CEP 8 tricolor dual-fusion probe. IGH (green) does not colocalise with MYC (red). The 8p11.1-q11.1 CEP8 alpha satellite probe gives a blue signal. (i) Colocalisation of BCL6 (green) and MYC (red) without fusion of IGH signals (blue).