Optimal Molecular Methods in Detecting p190^BCR-ABL Fusion Variants in Hematologic Malignancies: A Case Report and Review of the Literature

Figure 2

BCR-ABL1 qualitative RT-PCR amplification curves. Lightcycler amplification curves showing the cycle number of the crossing point (CP), the point where the reaction’s fluorescence reaches the maximum of the second derivative of the amplification curve corresponding to the point where the acceleration of the fluorescence signal is at its maximum. The CP is 29 cycles for the positive control (purple), 35 cycles for the patient’s peripheral blood specimen (grey), and no amplification for the negative control (no DNA) (green). Thus, the BCR-ABL1 fusion transcript was detected from the patient’s peripheral blood.