Serum Total Tryptase Level Confirms Itself as a More Reliable Marker of Mast Cells Burden in Mast Cell Leukaemia (Aleukaemic Variant)

Table 1

Primer positions according to the reference KIT sequence ENST00000288135 used for sequencing of KIT gene. mT: melting temperature.

Number of PCR	Product length	Sense primer	Position	mT	Antisense primer	Position	mT
1	426	CGAGAGCTGGAACGTGGAC	49	55,9	CCATACAAGGAGCGGTCAACA	474	56,2
2	359	GCACCAACAAACACGGCTTA	387	55,3	CACAGACACAACAGGCACAG	745	55,1
3	391	CCTACCATCGGCTCTGTCTG	630	55,3	ACTACTTCCAAGGTTGTTGTGA	1020	53,6
4	450	TGACTATCAGTTCAGCGAGAGT	921	54,8	TGCCATTCACGAGCCTGTC	1370	55,8
5	392	TCCCAAGTCTGAGAATGAAAGTAAT	1183	54,6	CCTTACATTCAACCGTGCCAT	1574	54,8
6	409	GGAAAGCTAGTGGTTCAGAGTTC	1505	55,4	CCTCAACAACCTTCCCGAAA	1913	53,7
7	385	ACATAGACCCAACACAACTTCC	1806	54,3	GCATGATCTTCCTGCTTTGAAC	2190	54,4
8	393	GCCCACCCTGGTCATTACA	2089	54,6	GCTGCCAAGTCTCTGTGAATAC	2481	55,5
9	393	CCTAGACTTAGAAGACTTGCTGAG	2386	54,7	TGTTCAGGGCTGAGCATCC	2778	55,0
10	420	GGTCGATTCTAAGTTCTACAAGATGA	2719	55,4	GGTGCCCACTATCCTGGAG	3138	54,5