KIT D816V Positive Acute Mast Cell Leukemia Associated with Normal Karyotype Acute Myeloid Leukemia

Figure 3

Bone marrow dot plots obtained by flow cytometry revealing 12% CD117+ CD34+ myeloid precursor cells (black dots), 5% CD117+CD34-mast cell precursors (orange dots), 13% of abnormal CD2− CD25+ mast cells (red dots), and 49% granulocytic cells with a maturation blockage at the promyelocyte level (purple dots). Myeloid precursor cells (black): CD117+, CD34+, CD2−, CD25−/+, CD13+, CD15−, CD16−, CD30−, CD33+, CD63+, CD65+low, CD69+low, CD123+, HLA-DR+high, and FcεRI/IgE−. Mast cell precursors (orange): CD117+, CD34−, CD2−, CD25+, CD13+, CD15−, CD16−, CD30+, CD33+, CD63+, CD65+low, CD69−, CD123+ high, HLA-DR−/+low, and FcεRI/IgE+. Mast cells (red): CD117+ high, CD34−, CD2−, CD25+ high, CD13+, CD15−, CD16−, CD30+, CD33+, CD63+, CD65+low, CD69+, CD123+ high, HLA-DR+high, and FcεRI/IgE+low.