Bone Marrow Aspirate Clot: A Useful Technique in Diagnosis and Follow-Up of Hematological Disorders

Figure 4

Case 3. The patient with final diagnosis of chronic lymphocytic leukemia (CLL). (a) BMC, in small magnification, showing intense hypercellularity of bone marrow with lymphoid accumulation (H&E, 40x). (b) Cell details, characterizing lymphocytes as small and typical of this neoplasia with hyperchromatic nuclei and regular membrane (H&E, 400x). (c) Immunopositivity for CD20, confirming B-cell origin of the neoplasia (IHC, CD20, 200x). (d) Contrast in lymphoid accumulation, showing a small percentage of T lymphocytes (IHC, CD3, 200x). (e) Immunopositivity overlapping for CD5 in neoplastic B cells (IHC, CD5, 200x). (f) Immunopositivity for CD23 allows diagnosis of CLL (IHQ, CD23, 200x). Note the quality of immunostaining in the images. In BMB samples, due to calcification, color irregularities can compromise analysis and make precise diagnostics sometimes difficult.

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