Case Report

Double-Hit Primary Plasma Cell Leukemia with IGH/MYC and IGH/CCND1 Translocations

Figure 2

Karyotype of metaphase chromosomes and interphase fluorescence in situ hybridization (FISH) analysis obtained from the bone marrow. (a) The following complex karyotypes were observed at diagnosis: 47, Y, t (X; 8; 14) (q24; q24; q32), t (11; 14) (q13; q32), and +18. The arrows indicate the abnormal chromosomes. (b) Additional karyotype aberrations, including der (11)t (11; 14) and der (14)t (8; 14), were observed after IRd therapy. (c) FISH analysis was performed using a vysis LSI IGH/CCND1 dual color, dual fusion translocation probe. The orange signal represents CCND1, and the green signal represents IGH. Four fusion signals were observed. (d) FISH analysis was performed using a vysis LSI IGH/MYC/CEP 8 tri-color dual fusion probe. The orange signal represents MYC, the green signal represents IGH, and the aqua signal represents alpha satellite sequences on 8q11.1. Two fusion signals were visible.
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