The hepatocellular adenoma is further subtyped as β-catenin activated HCA. (A) Immunostaining for glutamine synthetase shows diffuse reactivity within the tumor (200x), (B) β-catenin immunostain is negative for nuclear staining (400x). Intracytoplasmic globules in focal areas of the hepatocellular adenoma are observed. (C) PAS-D stain highlights small PAS positive and diastase resistant cytoplasmic globules (400x). These globules are immunoreactive with alpha-1-antitrypsin (D) but not alpha-1-antichymotrypsin (E) (400x).