Case Reports in Infectious Diseases

Case Reports in Infectious Diseases / 2012 / Article

Case Report | Open Access

Volume 2012 |Article ID 492467 |

Xenophon Sinopidis, Joseph Kaleyias, Konstantina Mitropoulou, Maria Triga, Sanjeev V. Kothare, Stefanos Mantagos, "An Uncommon Case of Pediatric Neurobrucellosis Associated with Intracranial Hypertension", Case Reports in Infectious Diseases, vol. 2012, Article ID 492467, 3 pages, 2012.

An Uncommon Case of Pediatric Neurobrucellosis Associated with Intracranial Hypertension

Academic Editor: S. Talhari
Received26 Mar 2012
Accepted21 Jun 2012
Published29 Jul 2012


We present the case of a 4-year-old boy who was admitted to hospital with intracranial hypertension, headache, diplopia, papilledema, and a normal brain MRI. Brucella melitensis in the cerebrospinal fluid was confirmed with PCR assay. We believe that neurobrucellosis should be included in the differential diagnosis when headaches persist following brucellosis. In addition, we suggest that when cerebrospinal fluid culture is negative, PCR may prove to be an optimal alternative tool for an immediate and accurate diagnosis.

1. Introduction

Brucellosis is an endemic zoonotic disease, common in certain areas such as the Mediterranean basin, the Middle East, or South America [1, 2]. During the last decades, globalization and technology made worldwide traveling easy. These rather recent population shifts introduced a new era in the epidemic profile of many infectious diseases, changing their distribution, and making them reappear in places where they were previously considered eradicated. For example in 1991, the United Kingdom was declared brucellosis-free yet 148 new cases were reported during the period 2001–2006 amongst them three affecting the central nervous system, otherwise called neurobrucellosis [3]. There are many discrepancies in the diagnostic criteria of neurobrucellosis, and the literature is mainly restricted to case reports and short series [2].

2. Case Presentation

A 4-year-old boy was admitted to our institution because of vomiting and persistent headaches. One month before admission, he had been admitted to another hospital with fever and difficulty walking. Brucellosis is endemic in the region, and Wright agglutination test is routinely performed among other examinations for the differential diagnosis of fever combined with limb dysfunction. The test was positive and the patient was treated by intramuscular gentamicin, oral doxycycline, and rifampicin. Poor compliance to oral antibiotics secondary to vomiting resulted in readmission to our hospital. CBC showed lymphocytic pleocytosis (WBC count 7.400/mL, polymorphonuclear cells 42%, and lymphocytes 48%). Erythrocyte sedimentation rate, liver and renal function tests were normal. The Wright agglutination test for brucellosis in the serum was positive at a titer of 1 : 320. Fundoscopy showed obliteration of the left optic disc margin. An antibiotic regimen of cotrimoxazole and rifampicin was initiated.

During the first week of admission, headaches worsened. Serial fundoscopy showed frank left side papilledema. Ocular movements revealed left abducens palsy and horizontal diplopia. Papillary light reflexes were bilateral normal and no afferent papillary defect was present. Meningeal or other focal neurological features were absent. Brain magnetic resonance imaging (MRI) was normal, and bone scanning did not reveal any focal involvement.

Lumbar puncture revealed clear cerebrospinal fluid (CSF) with high opening pressure (48 mmHg), and white blood cell count of 162 cells/mL (neutrophils 86%, monocytes 14%), glucose 32 mg/dL, and protein 60 mg/dL. CSF cultures were negative, but CSF polymerase chain reaction (PCR) examination was positive for brucellosis. Hence, the diagnosis of neurobrucellosis associated with intracranial hypertension was confirmed. Ciprofloxacin was added to the initial antibiotic regimen, and acetazolamide was administrated for intracranial hypertension.

Repeat fundoscopy ten days later showed improvement of the left-sided papilledema. A second measurement of intracranial pressure two weeks later showed an improvement at 33 mmHg, which was further reduced to 26 mmHg after removal of 10 mL of CSF fluid. Headache regressed gradually. One month after admission, fundoscopy showed residual blurring of the temporal margin of the left optic disc. Ciprofloxacin was substituted by moxifloxacine. Acetazolamide was well tolerated and was gradually withdrawn, based on clinical improvement and normal opening pressure measurement on the last lumbar puncture which was performed before discharge.

After two months in the hospital, the patient was discharged on a regimen of cotrimoxazole, rifampicin, and moxifloxacine for one month, followed by a period of four months with cotrimoxazole and rifampicin, with no residual deficits.

3. Discussion

Brucellosis is an endemic infectious disease of animals (zoonosis) that is transmittable to humans through the consumption of their products, after exposure to their blood, or through direct contact [1]. The symptoms of the infection are nonspecific, and often subtle [1]. Diagnosis is suspected if there is a high index of suspicion, due to history of animal product exposure or travelling [1]. A definitive diagnosis is obtained by isolation of Brucella species from the blood or other tissues, or by demonstration of high titers of specific antibodies in the serum [1].

Neurobrucellosis ranges from 1.7 to 10% of Brucella infections [4, 5]. Incidence in the pediatric age group is much lower, with an average rate of 0.8% [4, 5]. The most common clinical presentation is subacute and chronic meningoencephalitis [3, 7]. Myelitis, radiculoneuritis, brain and epidural abscess, and meningovascular syndromes are other inflammatory processes encountered [2]. MRI examination may present with normal findings, inflammatory changes, or white matter and vascular changes [8, 9].

Leukocyte pleocytosis and high protein levels are seen in the cerebrospinal fluid. Positive cultures from the serum and other specimens are observed in less than 50% [7, 10]. In a recent study of a large number of neurobrucellosis cases, only 28% of blood cultures and 14% of CSF cultures were positive for Brucella [2]. As Brucella is a slowly growing bacterium, initial cultures in earlier phases of the disease may be negative [3]. The detection of neurobrucellosis is often based on the neurological picture, evidenced by systemic Brucella infection, and the presence of inflammatory alteration in the CSF [2]. PCR assay of the cerebral fluid for Brucella is a novel evolving and promising diagnostic method [10]. PCR proved to be very helpful in the diagnosis of neurobrucellosis in our case, since CSF cultures were negative.

Involvement of one or more cranial nerves has been noted in more than 50% of neurobrucellosis cases [2, 11]. The main mechanism is basal meningitis affecting the course of the nerves. Other mechanisms include pseudotumor cerebri, vasculitis, and tetracycline side effects [2]. The vestibulocochlear nerve is the most frequently involved [2]. The long intracranial course of the abducens nerve makes it also susceptible to insults like microvascular infarction or direct compression [2]. One case of isolated abducens nerve palsy with borderline intracranial pressure in a young adult female patient has also been reported [11]. Facial, optic, and oculomotor nerves are other reported cranial nerves to be affected [2]. In a pooled analysis of 35 publications on neurobrucellosis in the general population, only one out of 187 (0.5%) patients presented with intracranial hypertension [2].

Papilledema is a common manifestation of intracranial hypertension. In our case this finding was unilateral. Unilateral papilledema is reported in cases of idiopathic intracranial hypertension [12]. The mechanism is unknown. A recent study suggests compartmentation of the subarachnoid space of the optic nerve [13]. Presentation of neurobrucellosis with increased intracranial pressure in children and adolescents is very uncommon (Table 1) [46, 14]. In a retrospective study of over 90 children with brucellosis, Tanir et al. reported a 13-year-old female patient with neurobrucellosis, increased intracranial pressure, negative CSF culture, and normal cranial MRI [6]. Yilmaz et al. reported recently a 15-year-old girl with headache, vomiting, diplopia with inward deviation of the left eye, and papilledema. Cranial MRI was normal. Intracranial hypertension was confirmed, and Brucella melitensis grew on CSF culture [5].

Sinopidis et al. Özisik et al. [4]Yilmaz et al. [5]Tanir et al. [6]

Patient gender and ageMale
4 years
38 years
15 years
13 years
Clinical presentationVomiting
Horizontal diplopia
Bilateral papilla stasis
Horizontal Diplopia
Intracranial hypertension, initially diagnosed as meningoencephalitis, no specific details given
Brain MRINormalNormalNormalNormal
Blood examinationsWBC count 7.400/mL
Neutrophils 42%
Lymphocytes 48%
Normal CRP and ESR
Wright agglutination test 1: 320
WBC count 5.500/mm3
Lymphocytes 64%, Neutrophils 24%
Monocytes 12%
ESR 20 mm/h
Brucella serum test 1/80
Normal blood count
Normal CRP
ESR 4 mm/h
Positive Rose Bengal test
Wright agglutination test 1: 160
Serum agglutination test 1: 320
2-Mercaptoethanol test 1: 160
Opening intracranial pressure48 mmHg300 mm H2O340 mm H2OIncreased, measurement not specified
CSF162 cells/mL
Neutrophils 86%
Monocytes 14%
Glucose 32 mg/dL
Protein 60 mg/dL
Negative culture
Positive PCR reaction
Lymphocytes 80/mm3
Protein 0.20 mg/L
Glucose 0.53 mg/L
Normal Gram and methylene blue stains
Negative ordinary culture
Positive BACTEC system culture
10 cells/μL
Glucose 58 mg/dL
Protein 48 mg/dL
Positive Rose Bengal test
Wright agglutination test 1: 80
Positive culture
500 cells/mm3
Lymphocytes 80%
Neutrophils 20%
Glucose 47 mg/dL
Protein 58 mg/dL
Serum agglutination test 1/10
Negative culture
TreatmentRifampicin, cotrimoxazole, ciprofloxacin added laterDoxycyclin, rifampicin, and trimethoprime with sulfamethoxazoleStreptomycin, doxycyclin, and rifampicinDoxycyclin, rifampicin, gentamicin

Our patient presented with intracranial hypertension, headache, diplopia, and papilledema, and normal brain-MRI. Brucella melitensis in the CSF was confirmed with PCR-assay. He is the youngest reported case with neurobrucellosis presenting the association of intracranial hypertension, diplopia, and normal cranial MRI. We suggest that neurobrucellosis should be included in the differential diagnosis when headaches persist following Brucellosis. In addition, we also suggest that when CSF culture is negative, PCR may prove to be an optimal alternative tool for an immediate and accurate diagnosis [10].

Conflict of Interests

The authors declare no conflict of interests.


  1. E. J. Young, “Brucellosis,” in Bacterial Infections of Humans, A. S. Evans and P. S. Brachman, Eds., pp. 177–188, Springer Science and Business Media, LLC, 2009. View at: Google Scholar
  2. H. C. Gul, H. Erdem, and S. Bek, “Overview of neurobrucellosis: a pooled analysis of 187 cases,” International Journal of Infectious Diseases, vol. 13, no. 6, pp. e339–e343, 2009. View at: Publisher Site | Google Scholar
  3. R. P. D. Cooke and N. J. Beeching, “Neurobrucellosis in the UK,” Journal of Infection, vol. 58, no. 1, pp. 88–89, 2009. View at: Publisher Site | Google Scholar
  4. H. I. Özisik, Y. Ersoy, M. Refik Tevfik, S. Kizkin, and C. Özcan, “Isolated intracranial hypertension: a rare presentation of neurobrucellosis,” Microbes and Infection, vol. 6, no. 9, pp. 861–863, 2004. View at: Publisher Site | Google Scholar
  5. S. Yilmaz, G. Serdaroglu, S. Gokben, and H. Tekgul, “A case of neurobrucellosis presenting with isolated intracranial hypertension,” Journal of Child Neurology, vol. 26, no. 10, pp. 1316–1318, 2011. View at: Google Scholar
  6. G. Tanir, S. B. Tufekci, and N. Tuygun, “Presentation, complications, and treatment outcome of brucellosis in Turkish children,” Pediatrics International, vol. 51, no. 1, pp. 114–119, 2009. View at: Publisher Site | Google Scholar
  7. N. Ceran, R. Turkoglu, I. Erdem et al., “Neurobrucellosis: clinical, diagnostic, therapeutic features and outcome. Unusual clinical presentations in an endemic region,” Brazilian Journal of Infectious Diseases, vol. 15, no. 1, pp. 52–59, 2011. View at: Publisher Site | Google Scholar
  8. O. Kizikilic and C. Calli, “Neurobrucellosis,” Neuroimaging Clinics of North America, vol. 21, no. 4, pp. 927–937, 2011. View at: Google Scholar
  9. M. W. Al-Sous, S. Bohlega, M. Z. Al-Kawi, J. Alwatban, and D. R. McLean, “Neurobrucellosis: clinical and neuroimaging correlation,” American Journal of Neuroradiology, vol. 25, no. 3, pp. 395–401, 2004. View at: Google Scholar
  10. J. D. Colmenero, M. I. Queipo-Ortuño, J. M. Reguera, G. Baeza, J. A. Salazar, and P. Morata, “Real time polymerase chain reaction: a new powerful tool for the diagnosis of neurobrucellosis,” Journal of Neurology, Neurosurgery and Psychiatry, vol. 76, no. 7, pp. 1025–1027, 2005. View at: Publisher Site | Google Scholar
  11. M. Yilmaz, R. Ozaras, A. Mert, R. Ozturk, and F. Tabak, “Abducent nerve palsy during treatment of brucellosis,” Clinical Neurology and Neurosurgery, vol. 105, no. 3, pp. 218–220, 2003. View at: Publisher Site | Google Scholar
  12. P. R. Wattamwar, N. N. Baheti, and A. Radhakrishnan, “Idiopathic intracranial hypertension presenting as unilateral papilledema,” Neurology India, vol. 58, no. 5, pp. 818–819, 2010. View at: Publisher Site | Google Scholar
  13. H. E. Killer, G. P. Jaggi, J. Flammer, N. R. Miller, A. R. Huber, and A. Mironov, “Cerebrospinal fluid dynamics between the intracranial and the subarachnoid space of the optic nerve. Is it always bidirectional?” Brain, vol. 130, no. 2, pp. 514–520, 2007. View at: Publisher Site | Google Scholar
  14. O. Tekeli, S. Tomac, E. Gursel, and H. Hasiripi, “Divergence paralysis and intracranial hypertension due to neurobrucellosis. A case report,” Binocular Vision and Strabismus Quarterly, vol. 14, no. 2, pp. 117–118, 1999. View at: Google Scholar

Copyright © 2012 Xenophon Sinopidis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

More related articles

 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.