Shigellosis Caused by CTX-M Type ESBL Producing Shigella flexneri in Two Siblings of Rural Nepal: First Case Report from the Country

Table 2

Primers for the bla-CTX-M, bla-TEM, and bla-SHV genes.

Gene	Primers (5′-3′)	Amplicon size (bp)
SHV	F: 5′-GTCAGCGAAAAACACCTTGCC-3′	383 bp
	R: 5′-GTCTTATCGGCGATAAACCAG-3′
TEM	F: 5′-GAGACAATAACCCTGGTAAAT-3′	459 bp
	R: 5′-AGAAGTAAGTTGGCAGCAGTG-3′
CTX-M	F: 5′-GAAGGTCATCAAGAAGGTGCG-3′	560 bp
	R: 5′-GCATTGCCACGCTTTTCATAG-3′