Methadone-Induced Delayed Posthypoxic Encephalopathy: Clinical, Radiological, and Pathological Findings
Figure 2
Cerebral cortex shows apparently normal neuronal density ((a) hematoxylin and eosin, ×200 original magnifications). The histological changes in the biopsy center in the white matter show increased cellularity with reactive gliosis ((b) hematoxylin and eosin, ×400; (c) anti-GFAP, ×200), microglial activation ((d) anti-CD68, ×200), and axonal changes, consistent with recent axonal injury ((e) anti-neurofilament, ×400; (f) anti-B-APP, ×200 all original magnifications) in the presence of abundant well-stained myelin ((g) luxol fast blue with hematoxylin and eosin, ×200). Ultrastructural evaluation confirms axonal degeneration with myelin preservation (h).
