Case Reports in Ophthalmological Medicine

Case Reports in Ophthalmological Medicine / 2016 / Article

Case Report | Open Access

Volume 2016 |Article ID 4148968 | 4 pages |

The Value of Cytology Smears for Acanthamoeba Keratitis

Academic Editor: Dipak Parmar
Received07 Jan 2016
Accepted17 May 2016
Published15 Jun 2016


Purpose. Acanthamoeba keratitis remains a difficult diagnosis despite advances in genetic and imaging technologies. The purpose of this paper is to highlight the utility of cytology smears for diagnosis of Acanthamoeba keratitis. Methods. This is a case study of the diagnostic course for a patient with suspected Acanthamoeba keratitis. Results. A 40-year-old male with poor contact lens hygiene presented with severe left eye pain. Slit lamp examination showed two peripheral ring infiltrates without an epithelial defect. The epithelium over both infiltrates was removed with a Kimura spatula. Half of the sample was smeared on a dry microscope slide and the other half was submitted for Acanthamoeba culture and PCR. Both culture and PCR were negative for Acanthamoeba, but hematoxylin and eosin stain of the smear revealed double-walled cysts. Conclusion. H&E staining of corneal cytology specimens is an efficient and readily available test for diagnosis of Acanthamoeba keratitis.

1. Introduction

Acanthamoeba keratitis is an uncommon cause of infectious keratitis that often goes undiagnosed until later stages of disease. The delay in diagnosis and appropriate treatment allows progression of disease with consequent visual morbidity. However, even when clinical findings suggest the diagnosis, confirmatory testing is challenging. Cultures for Acanthamoeba grow slowly and are often negative [1]. Although polymerase chain reaction (PCR) analysis of ocular specimens for Acanthamoeba has become more standardized, recent reports demonstrate limited utility compared to culture [2]. In vivo confocal microscopy of the cornea is useful for rapid and noninvasive identification of the double-walled cysts characteristic of Acanthamoeba, but instrumentation is expensive and not readily available [3]. We present a case to highlight the power of inexpensive, readily available cytology preparations for the rapid diagnosis of Acanthamoeba keratitis.

2. Case Presentation

A 40-year-old man with history of poor contact lens hygiene had onset of left eye redness, photophobia, irritation, and 8/10 pain. After a week of unsuccessful treatment with an unspecified antibiotic eye drop, his treatment was switched to moxifloxacin 4x/day and prednisolone acetate 1% 4x/day. He was referred to our clinic two weeks following the onset of symptoms. His presenting visual acuity was 20/20 in the right eye and 20/25 in the left eye. On examination, two peripheral ring infiltrates were present on his left cornea without any epithelial irregularity. Prednisolone eye drops were discontinued given the high clinical suspicion for Acanthamoeba keratitis. The following week, examination showed granularity of the epithelium overlying the ring infiltrates with an increase in stromal inflammation (Figure 1). The corneal epithelium over the infiltrates was removed with a Kimura spatula. Half of the sample was submitted for Acanthamoeba culture and PCR and the other half smeared directly on a dry microscope slide for routine cytology. The smear was allowed to air-dry. No fixative step was used.

Both Acanthamoeba culture and PCR were negative; however, hematoxylin and eosin (H&E) staining of the epithelial smear demonstrated the presence of double-walled cysts typical of Acanthamoeba keratitis (Figure 1, (B) and (C)). Another corneal epithelial debridement was performed and submitted for culture and cytology after 10 weeks of therapy with Chlorhexidine 0.02%, Neosporin, and Cyclopentolate 1% ophthalmic solutions. Culture remained negative; however, the cytology specimen again revealed the presence of double-walled cysts. Occasional single walled structures, representing single walled exocysts, were also seen. Electron microscopy studies indicate that exocystation is accompanied by at least partial enzymatic digestion of the endocyst [4]. This would explain the single wall appearance of the empty cysts. Also, such exocysts would not be expected to support growth in microbiological culture (as noted in this case) or contain genomic DNA for PCR detection.

Following 4 months of therapy with Chlorhexidine 0.02% and Neosporin, the patient’s eye pain decreased and the cornea showed stable stromal scarring. Therapy was gradually tapered without recurrence.

3. Discussion

In this case, high clinical suspicion and positive cytology allowed rapid diagnosis and initiation of appropriate therapy for Acanthamoeba keratitis. High clinical suspicion is essential for the diagnosis of Acanthamoeba keratitis with a positive predictive value of 89% [5]. However, confirmatory testing is necessary. Cytology services are present in most clinical centers and are therefore more accessible than facilities for in vivo corneal confocal microscopy, Acanthamoeba culture, or PCR. Cytological identification of the cyst does not require living organisms as needed for culture or intact DNA as required for PCR [6]. The value of cytology was well documented two decades ago during an outbreak of Acanthamoeba keratitis [7]. In that study, culture was negative in all of the specimens evaluated, but cytology was positive in over 80% of suspected cases. Almost half of cases equivocal for Acanthamoeba by in vivo confocal microscopy were positive on H&E stained cytology specimens. Other methods of staining that have been described for detection of Acanthamoeba include lactophenol-cotton blue, acridine orange, calcofluor white, and silver stain [8]. However, we decided to stain the smear with H&E as recent literature suggests that H&E is more sensitive and specific than other stains, particularly calcofluor white [9]. The cytological identification of Acanthamoeba has been underutilized in recent years perhaps due to the introduction of new molecular and imaging methods. In fact, there are only a limited number of descriptions of the cytological features of these organisms in smears [6, 10]. A summary of the pros and cons of diagnostic tests for Acanthamoeba keratitis is presented in Table 1.

Diagnostic modalityAdvantagesDisadvantages

Microbiological culture(i) Direct identification(i) Low sensitivity [1] 
(ii) Can take up to 1 week

Polymerase chain reaction (PCR)(i) Specific
(ii) Fast
(i) Requires intact DNA [2] 
(ii) Not readily available

In vivo confocal microscopy(i) Immediate identification of double-walled cysts
(ii) Noninvasive
(i) Not readily available
(ii) Requires trained observer to recognize cysts in images [1]

Histopathology(i) Specific
(ii) Requires several days for diagnosis
(iii) Multiple stains and/or immunoperoxidase studies can be done
(i) Requires significant tissue (corneal biopsy or keratoplasty specimen)

Cytological smear(i) Minimally invasive
(ii) Identifies both empty and double-walled cyst
(iii) Fast
(iv) Biopsy easy to perform
(i) Organisms in deep stroma not easily represented

Electron microscopy(i) Specific(i) Requires weeks to process
(ii) Expensive and labor intensive
(iii) Practical only for small tissue samples

Acanthamoeba keratitis requires a multifaceted diagnostic approach. The value of cytology for efficient diagnosis should not be underestimated. Close collaboration between the ophthalmologist and pathologist is key to obtaining an informative cytological study.


The opinions expressed herein do not necessarily represent those of the Veterans Administration or the US Government.

Competing Interests

The authors declare that there are no competing interests regarding the publication of this paper.


This work was funded in part by an unrestricted grant to the Department of Ophthalmology, SUNY-University at Buffalo, from Research to Prevent Blindness, New York, NY; facilities and resources provided by Kaleida Health, Buffalo, NY, and VAWNYHS; National Institutes of Health (R01 EY09412, Federico Gonzalez-Fernandez).


  1. E. Y. Tu, C. E. Joslin, J. Sugar, G. C. Booton, M. E. Shoff, and P. A. Fuerst, “The relative value of confocal microscopy and superficial corneal scrapings in the diagnosis of Acanthamoeba keratitis,” Cornea, vol. 27, no. 7, pp. 764–772, 2008. View at: Publisher Site | Google Scholar
  2. R. P. Kowalski, M. A. Melan, L. M. Karenchak, and A. Mammen, “Comparison of validated polymerase chain reaction and culture isolation for the routine detection of Acanthamoeba from ocular samples,” Eye & Contact Lens: Science & Clinical Practice, vol. 41, no. 6, pp. 341–343, 2015. View at: Publisher Site | Google Scholar
  3. E. Villani, C. Baudouin, N. Efron et al., “In vivo confocal microscopy of the ocular surface: from bench to bedside,” Current Eye Research, vol. 39, no. 3, pp. 213–231, 2014. View at: Publisher Site | Google Scholar
  4. B. Chávez-Munguía, M. Omaña-Molina, M. González-Lázaro, A. González-Robles, P. Bonilla, and A. Martínez-Palomo, “Ultrastructural study of encystation and excystation in Acanthamoeba castellanii,” Journal of Eukaryotic Microbiology, vol. 52, no. 2, pp. 153–158, 2005. View at: Publisher Site | Google Scholar
  5. M. A. Dahlgren, A. Lingappan, and K. R. Wilhelmus, “The clinical diagnosis of microbial keratitis,” American Journal of Ophthalmology, vol. 143, no. 6, pp. 940–944, 2007. View at: Publisher Site | Google Scholar
  6. Y. Sawada, C. Yuan, and A. J. W. Huang, “Impression cytology in the diagnosis of Acanthamoeba keratitis with surface involvement,” American Journal of Ophthalmology, vol. 137, no. 2, pp. 323–328, 2004. View at: Publisher Site | Google Scholar
  7. W. D. Mathers, J. E. Sutphin, R. Folberg, P. A. Meier, R. P. Wenzel, and R. G. Elgin, “Outbreak of keratitis presumed to be caused by Acanthamoeba,” American Journal of Ophthalmology, vol. 121, no. 2, pp. 129–142, 1996. View at: Publisher Site | Google Scholar
  8. J. Lorenzo-Morales, N. A. Khan, and J. Walochnik, “An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment,” Parasite, vol. 22, article 10, 2015. View at: Publisher Site | Google Scholar
  9. H. E. Grossniklaus, G. O. Waring IV, C. Akor, A. A. Castellano-Sanchez, and K. Bennett, “Evaluation of hematoxylin and eosin and special stains for the detection of Acanthamoeba keratitis in penetrating keratoplasties,” American Journal of Ophthalmology, vol. 136, no. 3, pp. 520–526, 2003. View at: Publisher Site | Google Scholar
  10. M. R. Kanavi, B. Hosseini, F. Javadi, N. Rakhshani, and M.-A. Javadi, “Impression cytology in eyes with clinical and confocal scan features of Acanthamoeba keratitis,” Journal of Ophthalmic and Vision Research, vol. 8, no. 3, pp. 207–212, 2013. View at: Google Scholar

Copyright © 2016 Sangita P. Patel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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