Case Reports in Pathology

Case Reports in Pathology / 2012 / Article

Case Report | Open Access

Volume 2012 |Article ID 138126 |

Rahul G. Matnani, Roshan K. Patel, Stephen E. Strup, Rouzan G. Karabakhtsian, "5q Minus Myelodysplasia Associated with Multiple Epithelioid Granulomas within Conventional Renal Cell Carcinoma", Case Reports in Pathology, vol. 2012, Article ID 138126, 4 pages, 2012.

5q Minus Myelodysplasia Associated with Multiple Epithelioid Granulomas within Conventional Renal Cell Carcinoma

Academic Editor: C. Gennatas
Received28 Feb 2012
Accepted10 May 2012
Published16 Jul 2012


A 69-year-old Caucasian female, with a previous diagnosis of 5q minus myelodysplastic syndrome, presented with conventional renal cell carcinoma (RCC) associated with multiple-epithelioid nonnecrotizing granulomas. Two previous reports of sarcoidosis, primarily involving the lung and skin, have been described in patients with 5q minus myelodysplasia. A cluster of closely linked genes encoding for cytokines such as IL-4, IL-5, and IL-3 are present on chromosome 5q. Hence, in sarcoidosis, cytokine imbalances associated with the deletion of these cytokine genes have been postulated. However, an occurrence of epithelioid granulomas within a carcinoma, in preexisting clonal myelodysplastic syndrome, has not been described. The patient, in the current study, had long standing 5q minus deletion, clinically characterized by refractory anemia associated with hypolobated megakaryocytes. However, the patient's history was negative for sarcoidosis and the extensive nonnecrotizing epithelioid granulomas were confined within RCC. Due to the absence of Th-2 cytokines, such as IL-4 and IL-5, in a subset of 5q minus myelodysplastic syndrome, proinflammatory Th-1 cytokines such as IFN-γ and TNF-α may be exaggerated in an environment conducive to antigen expression. Hence, we propose a greater susceptibility for the development of granulomas, at least in a subset of patients with 5q minus myelodysplasia.

1. Introduction

Ineffective hematopoiesis in myelodysplasia is characterized by hypercellular bone marrow and peripheral blood pancytopenia. The 5q deletion in myelodysplasia, in the absence of other complex cytogenetic abnormalities, is associated with a favorable prognosis or with a low risk for development of leukemia [1, 2]. Two previous reports linking 5q minus syndrome and sarcoidosis have been described [3, 4]. In both reports, sarcoidosis involved the lung and skin. However, three patients with a known history of nonclonal myelodysplasia have later developed disseminated granulomatous skin eruptions [5, 6]. Interestingly, multiple-gene encoding for cytokines such as IL-4, IL-5, and IL-3 and receptors for different growth factors such as platelet derived growth factor (PDGF) are closely linked on long arm of chromosome 5 [7, 8]. The dominant role of proinflammatory Th-1 cytokines such as IFN-γ and TNFα has been well documented in the pathogenesis of granuloma formation in various animal and human studies [912]. Hence, in absence of Th-2 cytokines (IL-4 and IL-5), cytokine imbalances associated with exaggerated Th-1 cytokines may occur in a subset of 5q minus myelodysplastic syndrome. This coupled with an environment favoring antigen expression, may lead to sustained macrophage and T-cell activation and subsequent granuloma formation. We report a case of multiple granulomas associated with RCC in a patient with 5q minus myelodysplasia.

2. Case Report

The patient is a 69-year-old lady with a previous diagnosis of clonal myelodysplasia, specific for 5q deletion, in the setting of refractory normocytic anemia. The bone marrow biopsy performed in 2007 showed small hypolobated megakaryocytes with bone marrow chromosomal analysis and fluorescence in situ hybridization (FISH) demonstrating deletion of 5q. The patient, however, was not treated with lenalidomide for 5q minus myelodysplastic syndrome due to previous history of pulmonary embolism and stroke. In addition, her past history was significant for hypertension, diabetes, and a history of brain aneurysm repair in 1995. The patient also reported allergy to sulfa drugs. The patient stopped smoking in 2002 and denied any alcohol use. The family history was negative for leukemia or myelodysplastic syndrome. In October 2011, she underwent a CT scan for left flank pain which revealed a left lower pole mass suspicious for RCC. At the time of surgery, in December 2011, the patient had refractory normocytic anemia with hemoglobin of 8.7 g/dL. However, all other hematological, biochemical, and serological parameters were normal. The patient did not have any history of sarcoidosis, tuberculosis, or history of immunization with Bacillus Calmette-Guérin (BCG). Clinical symptoms, additional imaging, and laboratory findings were negative for sarcoidosis.

3. Pathologic Findings

On gross examination, a fairly well-circumscribed tumor measuring  cm was present at the lower pole of the left kidney. The cut surface appeared yellow-red with a central area of hemorrhage. The tumor was confined to the kidney. The remaining uninvolved kidney was grossly unremarkable. Microscopically, the tumor showed features of conventional RCC of clear cell type with Fuhrman grade II nuclei (Figure 1). There was no evidence of lymphvascular invasion. Interestingly, there were multiple discrete epithelioid nonnecrotizing granulomas of variable size within the tumor (Figure 2). The granulomas demonstrated numerous multinucleated giant cells of foreign body type with lymphocytic infiltration (Figure 3). There was no evidence of asteroid or Schaumann bodies within the granulomas. The Gomori-Grocott methenamine silver stain and Fite’s acid fast stain did not reveal any fungal or mycobacterial organisms. The normal kidney parenchyma did not show any evidence of granulomas.

4. Discussion

Noncaseating granulomas in association with breast, liver, and colon carcinomas have been described [1315]. Non-necrotizing granulomatous reaction within the renal cell carcinomas (RCC) is rare with only a few published reports in the literature [1620]. The RCC described by Marinides et al. was of a papillary type. There is one case report of nonnecrotizing granulomas in RCC with sarcomatoid features [21]. However, RCC in other reports, similar to our case, had a clear cell pattern with the presence of epithelioid granulomas. The current case report may indicate a possible association between preexisting clonal myelodysplasia and granulomatous reaction within the RCC.

Our patient has a long-standing history of clonal (5q minus) myelodysplastic syndrome associated with refractory normocytic anemia and small hypolobated megakaryocytes. The granulomas were exclusively associated within the RCC tumor and were entirely absent in the surrounding nonneoplastic renal parenchyma. Previous case reports of granulomatous RCC in a preexisting myelodysplasia have not been reported. However, a few case reports of sarcoidosis involving lung and skin have been associated with 5q minus syndrome [3, 4]. In our case, the patient did not have any history or symptoms suggestive of sarcoidosis. Except for a long standing refractory anemia, the biochemical, serological, and hematological parameters were within normal limits. The cases reported by Tunkel et al. and Airaghi et al. along with our present case report suggest a possible association between epithelioid granulomas and 5q minus myelodysplastic syndrome. The possibility of this association is further strengthened based on the evidence of clustering of genes encoding for Th-2 cytokines on long arm of chromosome 5. Hence, deletion of these genes may lead to cytokine imbalance and initiation of granuloma formation upon antigenic stimulation. Moreover, nonclonal myelodysplasia has been shown to be rarely associated with disseminated nonnecrotizing granulomatous skin eruptions [5, 6]. Hence, propensity to develop noncaseating epithelioid granulomas, in the setting of myelodysplasia, warrants further investigation.

In conclusion, this is the first reported case of multiple granulomas confined to a renal cell carcinoma in a patient with preexisting myelodysplastic syndrome. The hypothesis of existence of cytokine imbalance and uncontrolled activation of T cells and macrophages, in the setting of 5q minus myelodysplasia has been previously proposed [3]. The present study provides further support to this hypothesis. However, unlike previous studies, these granulomas were confined to the renal cell carcinoma in the setting of preexisting blood dyscrasia. Further studies may potentially look more closely at the possible association of myelodysplasia and immune activation leading to formation of epithelioid granulomas.


  1. D. Haase, U. Germing, J. Schanz et al., “New insights into the prognostic impact of the karyotype in MDS and correlation with subtypes: evidence from a core dataset of 2124 patients,” Blood, vol. 110, no. 13, pp. 4385–4395, 2007. View at: Publisher Site | Google Scholar
  2. A. A. N. Giagounidis, U. Germing, and C. Aul, “Biological and prognostic significance of chromosome 5q deletions in myeloid malignancies,” Clinical Cancer Research, vol. 12, no. 1, pp. 5–10, 2006. View at: Publisher Site | Google Scholar
  3. A. R. Tunkel, K. J. Sebastianelli, N. Pandit, and J. I. Brody, “Development of 5q—myelodysplasia in a patient with sarcoidosis,” American Journal of Hematology, vol. 34, no. 3, pp. 225–227, 1990. View at: Publisher Site | Google Scholar
  4. L. Airaghi, D. Montori, F. Zorzi, A. Miadonna, and A. Tedeschi, “Sarcoidosis in a patient with 5q-myelodysplasia. A possible pathogenetic link between the two diseases,” Monaldi Archives for Chest Disease, vol. 55, no. 5, pp. 378–380, 2000. View at: Google Scholar
  5. J. P. Vestey, M. Turner, L. Biddlestone, K. McLaren, N. Goulden, and J. A. A. Hunter, “Disseminated cutaneous granulomatous eruptions associated with myelodysplastic syndrome and acute myeloid leukaemia,” Clinical and Experimental Dermatology, vol. 18, no. 6, pp. 559–563, 1993. View at: Publisher Site | Google Scholar
  6. K. A. Katz, “Disseminated cutaneous granulomatous eruption occurring in the setting of myelodysplasia,” Dermatology Online Journal, vol. 9, no. 4, p. 22, 2003. View at: Google Scholar
  7. M. J. Pettenati, M. M. Le Beau, and R. S. Lemons, “Assignment of CSF-1 to 5q33.1: evidence for clustering of genes regulating hematopoiesis and for their involvement in the deletion of the long arm of chromosome 5 in myeloid disorders,” Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 9, pp. 2970–2974, 1987. View at: Google Scholar
  8. J. Boultwood, C. Fidler, A. J. Strickson et al., “Transcription mapping of the 5q- syndrome critical region: cloning of two novel genes and sequencing, expression, and mapping of a further six novel cDNAS,” Genomics, vol. 66, no. 1, pp. 26–34, 2000. View at: Publisher Site | Google Scholar
  9. L. E. Layland, H. Wagner, and C. U. Prazeres da Costa, “Lack of antigen-specific Th1 response alters granuloma formation and composition in Schistosoma mansoni MyD88-/-mice,” European Journal of Immunology, vol. 35, no. 11, pp. 3248–3257, 2005. View at: Publisher Site | Google Scholar
  10. M. W. Ziegenhagen, M. E. Rothe, G. Zissel, and J. Müller-Quernheim, “Exaggerated TNFα release of alveolar macrophages in corticosteroid resistant sarcoidosis,” Sarcoidosis Vasculitis and Diffuse Lung Diseases, vol. 19, no. 3, pp. 185–190, 2002. View at: Google Scholar
  11. D. R. Roach, A. G. D. Bean, C. Demangel, M. P. France, H. Briscoe, and W. J. Britton, “TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection,” Journal of Immunology, vol. 168, no. 9, pp. 4620–4627, 2002. View at: Google Scholar
  12. L. H. Hogan, W. Markofski, A. Bock, B. Barger, J. D. Morrissey, and M. Sandor, “Mycobacterium bovis BCG-induced granuloma formation depends on gamma interferon and CD40 ligand but does not require CD28,” Infection and Immunity, vol. 69, no. 4, pp. 2596–2603, 2001. View at: Publisher Site | Google Scholar
  13. J. D. Coyne, “Colonic carcinoma with granulomatous (sarcoid) reaction,” Journal of Clinical Pathology, vol. 55, no. 9, pp. 708–709, 2002. View at: Publisher Site | Google Scholar
  14. D. Santini, G. Pasquinelli, M. Alberghini, G. N. Martinelli, and M. Taffurelli, “Invasive breast carcinoma with granulomatous response and deposition of unusual amyloid,” Journal of Clinical Pathology, vol. 45, no. 10, pp. 885–888, 1992. View at: Google Scholar
  15. H. Tomimatsu, M. Kojiro, and T. Nakashima, “Epithelioid granulomas associated with hepatocellular carcinoma,” Archives of Pathology and Laboratory Medicine, vol. 106, no. 10, pp. 538–539, 1982. View at: Google Scholar
  16. O. Hes, M. Hora, T. Vanecek et al., “Conventional renal cell carcinoma with granulomatous reaction: a report of three cases,” Virchows Archiv, vol. 443, no. 2, pp. 220–221, 2003. View at: Publisher Site | Google Scholar
  17. F. Campbell and A. G. Douglas-Jones, “Sarcoid-like granulomas in primary renal cell carcinoma,” Sarcoidosis, vol. 10, no. 2, pp. 128–131, 1993. View at: Google Scholar
  18. G. N. Marinides, I. Hajdu, and R. O. Gans, “A unique association of renal carcinoma with sarcoid reaction in the kidney,” Nephron, vol. 67, no. 4, pp. 477–480, 1994. View at: Google Scholar
  19. J. Kovacs, A. Varga, M. Bessenyei, and S. Gomba, “Renal cell cancer associated with sarcoid-like reaction,” Pathology and Oncology Research, vol. 10, no. 3, pp. 169–171, 2004. View at: Google Scholar
  20. V. B. Shah, P. Sharma, and H. R. Pathak, “Conventional clear renal cell carcinoma with granulomatous reaction,” Indian Journal of Pathology and Microbiology, vol. 53, no. 2, pp. 379–380, 2010. View at: Publisher Site | Google Scholar
  21. I. Piscioli, S. Donato, L. Morelli, F. Del Nonno, and S. Licci, “Renal cell carcinoma with sarcomatoid features and peritumoral sarcoid-like granulomatous reaction: report of a case and review of the literature,” International Journal of Surgical Pathology, vol. 16, no. 3, pp. 345–348, 2008. View at: Publisher Site | Google Scholar

Copyright © 2012 Rahul G. Matnani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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