A Fatal Case of Primary Basaloid Squamous Cell Carcinoma in the Intrahepatic Bile Ducts
Table 1
Antibodies and their corresponding clone, dilution, pretreatment, incubation methods, and source used for immunohistochemical analysis of the biopsies and resected tissue samples.
Antibody
Clone
Dilution
Pretreatment and incubation
Source
CK7
OV-TL 12/30
1 : 1000
1
Dako
CK20
KS20.8
1 : 400
1
Dako
CK17
E3
1 : 100
1
Dako
CK5/6
D5/16
1 : 20
1
Dako
P63
4A4
Ready to use (RTU)
1
Roche
Antihuman epithelial antigen (EP4)
Ber-EP4
1 : 25
2
Dako
Epithelial membrane antigen (EMA)
E29
1 : 50
1
Dako
MUC2
CCP58
1 : 150
1
Dako
MUC5AC
45M1
1 : 500
1
Neomarkers
CDX2
EPR2764Y
RTU
1
Roche
Villin
12D2C3
1 : 40
1
Dako
Polyclonal carcinoembryonic antigen (CEAP)
Poly
1 : 3000
1
Elektrabox
Hepatocyte specific antigen
OCH1E5
1 : 1000
1
Dako
Glypican
GC33
RTU
1
Roche
Vimentin
VIM 3B4
1 : 400
1
Dako
Synaptofysin
Dak-synapt
1 : 1500
1
Dako
Chromogranin
Poly
1 : 2000
1
Dako
Pretreatment and incubation. (1) HIER pH 8,5/32 min. (2) Protease 12 min/32 min.
