Renal Tubular Dysgenesis in a Case of Fetus Acardius Amorphus

Figure 3

Hematoxylin and Eosin (HE) staining and immunohistochemistry staining for villin, mucin 2 and calretinin in the intestine of the Fetus acardius amorphus. Villin is stained in red (ALEXA 568), mucin 2 in green (ALEXA 488) and nuclei in blue (Hoechst). Fetus acardius shows differentiated small (a) and large (b) intestine with positive apical villin staining (red) marking the intestinal brush border in cells of the crypt and villi and mucin 2 (green) positive Goblet cells in crypts and villi. Ganglion cells of the Auerbach and Meissner Plexus were also present shown by positive Calretinin staining. Images are shown in 10x and in 20x magnifications.