Primary Signet Ring Cell/Histiocytoid Carcinoma of the Eyelid: Clinicopathologic Analysis with Evaluation of the E-Cadherin/β-Catenin Complex and Associated Genetic Alterations

Table 2

Immunohistochemical results for four SRC/Histiocytoid carcinomas of the eyelid.

Antibody	Case 1	Case 2	Case 3	Case 4
CK7	Positive	Positive	Positive	Positive
CK20	Negative	Negative	Negative	Negative
p63	Negative	Negative	Negative	Negative
p53	Negative	Negative	Negative	Positive^+++
E-cad	Negative^a	Negative^a	Positive	Positive
β-Catenin	Abnormal^b	Abnormal^b	Positive	Positive
Src	Low^c	Low^c	High^d	High^d
c-Myc	Negative	Negative	Negative	Positive^+
Cyclin-D1	Positive^++	Negative	Positive^+	Positive^+
GCDFP-15	Not performed	Positive^f	Positve^f	Positive^f
AR	Not performed	Positive	Not performed	Positive
Her2Neu	Not performed	Score 2+^e	Score 1+	Score 1+

^aNegative: <10% of the tumor cells staining for E-cadherin. ^bAbnormal: heterogeneous, incomplete membranous staining for B-catenin. ^cDecreased/low Src cytoplasmic staining in less than 25% of tumor cells. ^dHigh cytoplasmic Src expression in 25%-75%of tumor cells. ^eHer2 FISH was negative. ^fPerformed in an outside facility. ⁺Focal positivity (1%-<25% of tumor cells). ⁺⁺Moderate positive expression in >25%-<50% of tumor cells. ⁺⁺⁺Strong positive immunoreactivity seen in >50% of tumor cells.