Artificially Positive Crossmatches Not Leading to the Refusal of Kidney Donations due to the Usage of Adequate Diagnostic Tools
Table 1
(a) CDC- and AMS-ELISA -based crossmatch results of the 41-year-old recipient and her 60-year-old mother as a prospective living kidney donor. (b) Corresponding analyses for anti-HLA antibody screening/identification.
(a)
Serum sample
CDC-CM (#)
AMS-ELISA-CM
PBL
T-cell
B-cell
Class I
Class II
05/2010
1
1
1
n.d.
n.d.
10/2010
2/4
1/2
4
neg.
neg.
01/2011
1
1
1
neg.
neg.
n.d.: not done due to the unequivocally negative outcome of the CDC-CM; neg.: negative; pos.: positive; #: NIH score system of the standard CDC-based crossmatch as a percent of positive/dead (red coloured) cells (%): 1: <10% (negative), 2: 10–20% (doubtfully positive), 4: 20–50% (weakly positive), 6: 50–80% (positive), 8: 80–100% (strongly positive).
(b)
Serum sample
Screening ELISA
Luminex analysis
Class I (Quik Screen)
Class II (B-Screen)
Class I
Class II
05/2010
neg.
neg.
n.d.
n.d.
07/2010
neg.
neg.
n.d.
n.d.
10/2010
neg.
neg.
neg.
neg.
01/2011
neg.
neg.
neg.
neg.
n.d.: not done; neg.: negative; the Luminex analysis was not performed using the samples taken in May 2010 and July 2010 due to the unequivocally negative result of the class I and II screening ELISA but was additionally used to analyse the sera taken in October 2010 and January 2011.
