Case Report | Open Access
Enucleation in a Cownose Ray (Rhinoptera bonasus)
Trauma is a common problem in Cownose Ray during mating season in both wild and captive rays. Enucleation is indicated when there is an ocular trauma. A 5-year-old female Cownose Ray (Rhinoptera bonasus) from Aquaria of Kuala Lumpur Convention Centre (KLCC) was presented to University Veterinary Hospital (UVH), Universiti Putra Malaysia, with a complaint of protruding left eye, which resulted from crushing into artificial coral during mating season. There were a hyphema in the traumatic left eye, periorbital tissue tear, exposed left eye socket, and multiple abrasions on both pectoral fins. The Cownose was anaesthetized and maintained with isoeugenol and on-field emergency enucleation of the left eye was performed. It was managed medically with postoperative enrofloxacin, tobramycin ointment, and povidone iodine. No suture breakdown and secondary infection were observed at day 7 after enucleation during revisit. At day 24 after enucleation, the Cownose responded well to treatment with excellent healing progression and no surgical complication was observed.
The Cownose Ray (Rhinoptera bonasus) is also known as Cowfish or Skeete. They are classified as “Near Threaten” due to low productivity and as bycatch with pounds net. Schools of Cownose Ray usually occurred in estuaries, in shore, and in open ocean and can be found in Western Atlantic Ocean, Gulp of Mexico, and part of Caribbean Sea . They gain their name from their unique forehead which resembled the nose of cattle . Cownose Ray is one of the 42 species in the Myliobatidae family under the Elasmobranchii order and the cartilaginous fish including sharks and skates. As carnivores, their diet is consisted with bottom-dwelling shellfish, lobsters, carbs, and fish. Electroreceptors on snout are an excellent sense to touch and smell aids them to locate their prey .
Mating behaviors can be observed in the wild from early October to late June but primarily between April and June, when male-inflicted bites wounds on female pectoral fins are commonly observed . Trauma is also a common problem in captive rays during breeding season, including ocular trauma, and nonfatal external injuries. Furthermore, there is a likelihood of internal injuries which sometimes causes death . Therefore, the objective of the case report is to treat the ocular trauma by adopting a surgical approach such as enucleation to accomplish the desired response.
2. Case Report
A 5-year-old female Cownose (Rhinoptera bonasus) was managed in Oceanarium, the large scale exhibition area of Aquaria in Kuala Lumpur (KLCC) with over 40 species of elasmobranchs and teleost fish. She is fed with marine chopped fish twice a day and Mazuri supplement (vitamins and minerals).
Cownose’s caretaker noticed her left eye ball is protruding out from the orbit. He suspected she had crushed into the artificial coral when the male rays chased her for mating, and the period correlates with the mating season. The case was presented to University Veterinary Hospital (UVH), Universiti Putra Malaysia, on the same day that he complained of the eye trauma during field visit to Aquaria KLCC.
Physical examination was carried out. The Cownose was alert and responsive as she was actively swimming in the holding area. She was weighing 8 kg and had a Body Condition Score of 3 out of 5 and less than 5% dehydration. Temperature and pulse rate were not obtained, while the respiratory rate was 65 breaths per minutes. The left eye globe with hyphema was protruded from the orbital space and attached to the optic nerve (Figure 1). The pupillary reflex could not be assessed due to hyphema. The left orbital space was exposed and periocular tissue tear was present around the left orbit (Figure 2). Besides, multiple abrasions were observed at the cranial margin of the both pectoral fins, which was more severe on left fin.
Immersion anaesthesia was opted. Seventeen ppm of isoeugenol (brand name: Aqui-s) was used as anaesthetic agent for both induction and maintenance of surgical plane. The anaesthetic bath was prepared by filling a tank with 475 L of artificial sea water from the holding area and added with 14.8 mL of Aqui-s. Ventilation rate was the only parameter that was used to monitor the anaesthesia depth of the Cownose. The normal ventilation rate was taken at rest before induction, which will be used as the baseline to monitor ventilation throughout the anaesthesia . Ventilation rate of Cownose was taken by observing movement of spiracular flap and recorded every 5 minutes.
The Cownose was left in the anaesthetic bath for 10 minutes until its ventilation rate ceased to 40 breaths per minute and dropped in pectoral fin stroke activity (Figure 3). Later she was transferred by using canvas hammock to surgical tank with wet towel at the bottom and covered with another wet towel on her dorsum to maintain skin moisture  (Figure 4). The maintenance of surgical plane and establishment of rebreathing system was done by flushing water with anaesthetics agent through the gills via right spiracle (respiratory opening caudal to eye) with a 20 mL syringe.
The Cownose was presented in ventral recumbency and assistants aided in restraining by gently exerting force at the dorsum as a part of tonic immobilization. Routine skin preparation around the left eye was done by using diluted chlorhexidine gluconate (dilution ratio 1 : 30) and diluted povidone iodine (dilution ratio 1 : 30) .
A ring block was done around the left eye by using 5 mL 20 mg/mL Lidocaine as local anaesthesia to reversibly desensitize the skin as well as analgesia (Figure 5). Diluted gentamycin (5 mL, 100 mg/mL gentamycin + 5 mL sterile water) was prepared to flush the left orbital space and globe to minimize secondary infection since sterile surgical field is impossible to be established in the field setting (Figure 6). The optic nerve was clamped by using 2 Rochester Pean forceps (Figure 7). A surgeon knot was placed proximal to the first forceps with 2.0 PDS to ligate the blood vessel for haemostasis. Then the optic nerve was transected between the 2 forceps by using scalpel blade #20 (Figure 8). The globe, the remaining conjunctivae fat, and extraocular muscle tissue were removed. The pedicle was checked for presence of bleeding before releasing it into the orbital space. The inner and outer muscular attachments were sutured with 2.0 PDS using interrupted suture pattern to close the orbital space (Figure 9). Periorbital skin closure was done with 2.0 PDS in interrupted suture pattern (Figure 10).
The enucleation procedure took 15 minutes to be completed. Fresh sea water without an anaesthetic agent was used to flush through the spiracle to perfuse the gills and accelerate recovery from anaesthesia. The recovery from anaesthesia was indicated by increasing ventilation rate to preinduction rate, which was 66 bpm in Cownose and when she started struggling actively by flapping pectoral fins at 25th minutes.
Postoperatively, 5 mg/kg enrofloxacin was administered intramuscularly at the dorsolateral musculature close to the spine and terramycin ointment (active component: oxytetracycline hydrochloride) was applied twice a day topically on the suture site to prevent secondary infection of the suture site, orbital space, and optic sulcus . Besides, the caretaker was instructed to apply povidone iodine (brand name: Betadine) on the abrasions of the pectoral fins twice a day until wound healed to prevent the proliferation of Fusarium sp., an environment saprophytic fungi [8–10].
Client education consisted of isolating the Cownose from the school in holding area until surgical wound healed before releasing it to the large exhibition area, for ease of monitoring and for the prevention of possible infection at suture site as well as for administering daily treatment . Besides, daily monitoring of suture site for break and possible signs of secondary infection such as discolouration of skin, fungal growth on the wound, inappetence, and loss of body condition is recommended by Mylniczenko and Penfold . Next, putting of the mating pairs into separate tanks during mating season is necessary and a good practice. The mating process of rays is always aggressive as female rays always are being bitten and injured by the male rays, in which death can occur in severe case . Others include reducing risk of crushing into decorations; isolation also reduces the stress of mating animals by avoiding being attacked by other fish during and after mating.
At day 7 after operation, we had a revisit trip to Aquaria KLCC for the follow-up. She was responding well to the treatment with good appetite and no clinical abnormalities were observed, and suture materials were still intact (Figure 11). The last follow-up was on day 24 after operation and was done by phone; Cownose was having good appetite and the scars were returning to normal skin colour.
According to Mylniczenko and Penfold , trauma is a common problem in ray which occurred due to aggressive mating behaviour. The male-inflicted injuries such as ocular damage, fin damage, and ulceration of ventral skin could be commonly observed during mating season. A study done on reproductive biology of Cownose Ray in wild at Charlotte Harbor Estuarine System, Florida, divulged the presence of bite wound and scars on pectoral fins of female Cownose Ray which can be observed between April and June as well as observation of group of male rays chasing female rays for mating . However, no ocular damage was reported from the study. Besides, ocular trauma due to blunt trauma can be seen in Cownose rays. This is due to the lack of anatomical structures for protection of the globe, such as partially exposed globe from the cranium and absence of eyelids . In the present case report, Cownose was kept in a captive environment with a lot of artificial decorations and obstacles. Therefore she could be potentially crushed into these objects when chased by male rays. In short, ocular damage due to blunt trauma is possible in an aquarium setting.
A safe and effective anaesthetic protocol can contribute to a successful surgical outcome . Injectable anaesthesia such as ketamine  can be employed in sharks. Advantage of injectable anaesthesia was to allow the capture of animal in large exhibits, but no injectable anaesthesia was reported in rays . There were also a few choices of immersion anaesthesia agent reported to be used in rays , while the usage of MS-222 is the most common [16, 17]. However, MS-222 is acidic in aqueous solution with pH of approximate 3 and must be evaluated in marine system before induction although salt water contains adequate inherent buffering . Eugenol (Clove oil) can result in rapid induction and prolonged recovery when compared to MS-222 but has a narrow margin of safety which may be seen as respiratory failure in higher dose . Isoeugenol (brand name: Aqui-S) which is structurally similar to eugenol was effective at low concentration compared to eugenol and has less adverse reaction , although it will caused impaired ventilation and cardiovascular depression . In the present case report, we selected isoeugenol because it was relatively easier to be prepared in an on-field operation and caused less stress to Cownose. Throughout the anaesthesia, although we observed mild reduction of ventilation rate, no any detrimental effect was observed in the Cownose. Therefore, isoeugenol is safe and can be considered as a good choice for immersion anaesthesia of Cownose Ray.
The administration of postoperative antibiotics is controversial as in mammalian patient and shall not be a replacement for poor aseptic techniques due to emerging of antimicrobial resistance reported in many species . However, the justification for the present case was the limitation to establish an aseptic surgical field in such an on-field situation. In addition, our patient was for exhibition purpose but not for human consumption. By referring to Elasmobranch Husbandry Manual that was published by Ohio Biological Survey 2004, a few drug of choice had been described. Bath is a common technique but medicated water must be disposed carefully in accordance with regulations; meanwhile some oral medications can be rejected due to the unusual taste . Parental administration (intramuscular injection) was suitable in the present case report because it can be performed during the recovery period, and this procedure ensures that the animal received the correct dosage and reduces drug wastage. Enrofloxacin, the quinolone class, was selected because it has a broad spectrum activity against most gram-negative and many gram-positive bacteria. Besides, it was reported not to have an adverse effect on cartilage and its half-life in the plasma is 114 hours after injection probably due to its slow drug metabolism and excretion in the elasmobranch .
Vision does play an important role in most of the predatory behaviour, which is associated with the presence of a binocular vision . However in the Cownose’s case, the removal of the left eye would affect her predatory behaviour. Rays had a poor vision as compared to other senses such as olfactory and taste . From the anatomical aspect, ray’s eyes were positioned on the dorsal side, which is the opposite of their mouth that is located at the ventral side. Therefore, they must rely on the senses other than vision in foraging . Besides, rays did possess an anterior binocular convergence but the overlapping area was much less compared to other predators. This implicated that ray’s vision was not the primary sensory organ that aided in hunting . Lastly, there is one powerful sensory adaptation present in all elasmobranch that consisted of multiple small subcutaneous vesicles located around the head, snout, or mouth area. The ampullae of Lorenzini allowed elasmobranchs to detect electric fields from a prey and response to mechanical stimuli, even if vision and olfactory cues were absent . Ocular damage can occur in a female Cownose Ray during mating season in aquarium setting resulting from avoidance from being mated by aggressive male rays, limited space, presence of plenty artificial decorations, and anatomical structure of the forehead [5, 13]. Enucleation is the best option for case of severe ocular trauma if the traumatized eye was not viable . This procedure also yielded a good outcome because impaired vision was not necessary to affect predatory behaviour of a Cownose Ray . Next, judicious antibiotics administration in ray is crucial as a part of postoperative management to prevent secondary wound infection . Finally, management of mating animals during mating season is the key of reducing mating behaviour associated mortality in captive environment .
In conclusion, enucleation did not compromise the hunting behaviour and the patient’s quality of life. Therefore, surgical approach such as enucleation has the desired efficacy in the present case report.
Conflicts of Interest
The authors declare that they have no conflicts of interest.
The authors wish to thank Dr. Fuad Matori, Dr. Nur Asyikin Haron, Ms. Tuan Nur Mahiran, and Mr. Syafiq Abd Rahaman and acknowledge University Veterinary Hospital (UVH), Faculty of Veterinary Medicine, Universiti Putra Malaysia, and Aquaria KLCC for their technical assistance.
- A. S. Barker, Rhinoptera bonasus, 2006, The IUCN Red List of Threatened Species 2006: e.T60128A12310195. http://dx.doi.org/10.2305/IUCN.UK.2006.RLTS.T60128A12310195.en.
- Cownose Ray Facts. Saint Louis Zoo. Retrieved 25 September 2017, from https://www.stlzoo.org/visit/thingstoseeanddo/stingraysatcaribbeancove/cownoserayfacts/.
- M. Edmonds, “How do sharks see, smell and hear?” http://animals.howstuffworks.com.
- R. M. Scharer, W. F. Patterson III, J. K. Carlson, and G. R. Poulakis, “Age and Growth of Endangered Smalltooth Sawfish (Pristis pectinata) Verified with LA-ICP-MS Analysis of Vertebrae,” PLoS ONE, vol. 7, no. 10, Article ID e47850, 2012.
- N. Mylniczenko and L. M. Penfold, “Ultrasound findings in southern stingrays (Dasyatis americana) with and without reproductive disease,” in Proceedings of the 43rd Annual IAAAM Conference, Bay Lake, FL, USA, 2012.
- D. L. Neiffer and M. A. Stamper, “Fish sedation, anesthesia, analgesia, and euthanasia: Considerations, methods, and types of drugs,” ILAR Journal, vol. 50, no. 4, pp. 343–360, 2009.
- K. K. Sladky and E. O. Clarke, “Fish Surgery,” Veterinary Clinics of North America: Exotic Animal Practice, vol. 19, no. 1, pp. 55–76, 2016.
- G. A. Lewbart, “Fish,” in Exotic Animal Formulary, J. W. Carpenter, Ed., pp. 38–78, Elsevier Inc, Missouri, 2nd edition, 2013.
- N. Fernando, S.-W. Hui, C.-C. Tsang et al., “Fatal Fusarium solani species complex infections in elasmobranchs: The first case report for black spotted stingray (Taeniura melanopsila) and a literature review,” Mycoses, vol. 58, no. 7, pp. 422–431, 2015.
- M. M. Garner, “A Retrospective Study of Disease in Elasmobranchs,” Veterinary Pathology, vol. 50, no. 3, pp. 377–389, 2013.
- M. L. Larsson, “Binocular vision, the optic chiasm, and their associations with vertebrate motor behavior,” Frontiers in Ecology and Evolution, vol. 3, 2015, https://doi.org/10.3389/fevo.2015.00089.
- G. R. Poulakis, “Reproductive Biology of the Cownose Ray in the Charlotte Harbor Estuarine System, Florida,” Marine and Coastal Fisheries, vol. 5, no. 1, pp. 159–173, 2013.
- B. R. Whitaker, “Ocular Disorders,” in BSAVA Manual of Ornamental Fish, W. H. Wildgoose, Ed., pp. 147–154, BSAVA, 2001.
- M. T. Sally, “Hyphaema,” in Saunders Solution in Veterinary Practice: Small Animal Opthalmology, F. Nind, Ed., p. 36, Elsevier, 2008.
- M. A. Stamper, S. M. Miller, and I. K. Berzins, “Pharmacology in Elasmobranchs,” in Elasmobranch Husbandry Manual: Captive Care of Sharks, Rays, and their Relatives, M. Smith, D. Warmolts, D. Thoney, and R. Hueter, Eds., pp. 447–466, Ohio Biological Survey Inc, Ohio, 2004.
- R. H. George, J. Steeil, and K. Baine, “Ovariectomy of Sub-adult Southern Stingrays (Dasyatis americana) to Prevent Future Reproductive Problems,” in Proceedings of the Proceedings IAAAM, vol. 12, 2013, http://www.vin.com/doc/id.
- D. M. McComb and S. M. Kajiura, “Visual fields of four batoid fishes: a comparative study,” Journal of Experimental Biology, vol. 211, no. 4, pp. 482–490, 2008.
- S. Javahery and A. H. Moradlu, “AQUI-S, A New Anesthetic for Use in Fish Propagation,” Global Veterinaria, vol. 9, no. 2, pp. 205–210, 2012.
- L. U. Sneddon, “Clinical Anesthesia and Analgesia in Fish,” Journal of Exotic Pet Medicine, vol. 21, no. 1, pp. 32–43, 2012.
- R. Davis, “Quarantine and Prophylaxis for Elasmobranchs,” in Elasmobranch Husbandry Manual: Captive Care of Sharks, Rays, and their Relatives, M. Smith, D. Warmolts, D. Thoney, and R. Hueter, Eds., pp. 143–150, Ohio Biological Survey, Inc., Ohio, 2004.
- K. Knight, “How stingrays sense their surroundings,” Journal of Experimental Biology, vol. 212, no. 19, 2009.
- Fisheries and Oceans Canada, (2016), Government of Canada, Retrieved 17 September 2017, from http://www.dfo-mpo.gc.ca/species-especes/skates/anatomy/external-eng.html.
- W. H. Wildgoose and G. A. Lewbart, “Therapeutics,” in BSAVA Manual of Ornamental Fish, W. H. Wildgoose, Ed., pp. 237–258, BSAVA, 2001.
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