Research Article

ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome

Figure 2

Validation of serum HO-1 ELISA. (a) In a typical standard curve using modified ELISA, the correlation coefficient from 7 standard curves was 0.998 ± 0.002. The lower limit of detection was 0.038 ng/mL. (b) Comparison shows that the average serum HO-1 of 15 healthy samples was 17.2 ± 9.5 ng/mL by modified ELISA (all with measurable concentrations) and 2.1 ± 0.7 ng/mL by commercial ELISA (undetectable concentrations in 4 of 15 samples). ELISA, enzyme-linked immunosorbent assay; HO-1, hemeoxygenase-1.
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