Research Article

Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

Table 2

List of ELISA tests, cell proliferation, and protein extraction kits used. For ELISA tests, dilution of the supernatants or cell lysate samples used and detection limits are also reported.

TargetSupplierCat. #aDilutionDetection limit (range)

c-Kit or SCFR (CD117)Cloud-Clone Corp.SEA121 Hu1 : 5 (PBS)0.61 ng/mL (1.56–100 ng/mL)
PCNACloud-Clone Corp.SEA591Mi1 : 5 (PBS)0.055 ng/mL (0.156–10 ng/mL)
Thy1 (CD90)Cloud-Clone Corp.SEB404 HuNo dil5.3 pg/mL (12.5–800 pg/mL)
TGFβ1Cloud-Clone Corp.SEA124 HuNo dil.5.8 pg/mL (15.6–1000 pg/mL)
Procollagen-1Cloud-Clone Corp.SEA957 HuNo dil.12.3 pg/mL (31.2–2000 pg/mL)
NF-κB-p65InvitrogenKHO03711 : 5 (diluent buffer)<17pg/mL (0–5000 pg/mL)
WST-1 cell proliferationAbnovaKA1384////
M-PER mammalian protein extractionThermo Scientific78501////