Research Article

MicroRNA-101-3p Suppresses mTOR and Causes Mitochondrial Fragmentation and Cell Degeneration in COPD

Figure 3

The effect of TNF-α on ASMC miR-101-3p expression and mitochondria. (a) Bars show TNF-α upregulated miR-101-3p expression in ASMC from healthy nonsmokers (control) and COPD-ASMC (n = 3/group). values present comparison to time point 0. (b) Western blots for mitochondrial cytochrome c oxidase subunit II (MTCO2) degradation by TNF-α in ASMC of healthy nonsmokers (control, n = 3) and COPD-ASMC (n = 3). Bars present mean ± S.E.M. of image analysis data. values present comparison to time point 0. (c) Z-stack projection photos (Nikon Confocal, 60x magnification) of mitochondrial fragmentation, induced by TNF-α in ASMC of healthy nonsmokers (control) and COPD-ASMC. Mitochondria are indicated by staining of cytochrome c (green). (d) Image analysis of mitochondrial fragmentation is presented in Figure 3(c). Bars represent mean ± S.E.M. of cells with fragmented mitochondria, determined by double blind counting.
(a)
(b)
(c)
(d)