Table of Contents Author Guidelines Submit a Manuscript
Cardiology Research and Practice
Volume 2009, Article ID 802373, 9 pages
http://dx.doi.org/10.4061/2009/802373
Research Article

Two-Photon Laser Scanning Microscopy of the Transverse-Axial Tubule System in Ventricular Cardiomyocytes from Failing and Non-Failing Human Hearts

1Department of Medicine, Institute of Molecular Cardiobiology, Johns Hopkins University, Baltimore, MD 21205, USA
2Department of Cardiology and Pneumology, Georg-August-University Göttingen, 37075 Göttingen, Germany
3Department of Physiology, Cardiovascular Research Center, Temple University School of Medicine, Philadelphia, PA 19140, USA
4Department of Cardiology, University of Bonn, 53105 Bonn, Germany
5Department of Medicine, University School of Medicine, Durham, NC 27705, USA

Received 7 August 2009; Accepted 17 November 2009

Academic Editor: Chim C. Lang

Copyright © 2009 Andreas Ohler et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. D. M. Bers, “Cardiac excitation-contraction coupling,” Nature, vol. 415, no. 6868, pp. 198–205, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. G. A. Langer and A. Peskoff, “Calcium concentration and movement in the diadic cleft space of the cardiac ventricular cell,” Biophysical Journal, vol. 70, no. 3, pp. 1169–1182, 1996. View at Google Scholar · View at Scopus
  3. G. Hasenfuss, “Alterations of calcium-regulatory proteins in heart failure,” Cardiovascular Research, vol. 37, no. 2, pp. 279–289, 1998. View at Publisher · View at Google Scholar
  4. G. Hasenfuss and B. Pieske, “Calcium cycling in congestive heart failure,” Journal of Molecular and Cellular Cardiology, vol. 34, no. 8, pp. 951–969, 2002. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Schaper, R. Froede, S. Hein et al., “Impairment of the myocardial ultrastructure and changes of the cytoskeleton in dilated cardiomyopathy,” Circulation, vol. 83, no. 2, pp. 504–514, 1991. View at Google Scholar · View at Scopus
  6. R. R. Kaprielian, S. Stevenson, S. M. Rothery, M. J. Cullen, and N. J. Severs, “Distinct patterns of dystrophin organization in myocyte sarcolemma and transverse tubules of normal and diseased human myocardium,” Circulation, vol. 101, no. 22, pp. 2586–2594, 2000. View at Google Scholar · View at Scopus
  7. J. Q. He, M. W. Conklin, J. D. Foell et al., “Reduction in density of transverse tubules and L-type Ca2+ channels in canine tachycardia-induced heart failure,” Cardiovascular Research, vol. 49, no. 2, pp. 298–307, 2001. View at Google Scholar
  8. R. C. Balijepalli, A. J. Lokuta, N. A. Maertz et al., “Depletion of T-tubules and specific subcellular changes in sarcolemmal proteins in tachycardia-induced heart failure,” Cardiovascular Research, vol. 59, no. 1, pp. 67–77, 2003. View at Publisher · View at Google Scholar · View at Scopus
  9. J. A. Mattiello, K. B. Margulies, V. Jeevanandam, and S. R. Houser, “Contribution of reverse-mode sodium-calcium exchange to contractions in failing human left ventricular myocytes,” Cardiovascular Research, vol. 37, no. 2, pp. 424–431, 1998. View at Publisher · View at Google Scholar
  10. K. Dipla, J. A. Mattiello, K. B. Margulies, V. Jeevanandam, and S. R. Houser, “The sarcoplasmic reticulum and the Na+/Ca2+ exchanger both contribute to the Ca2+ transient of failing human ventricular myocytes,” Circulation Research, vol. 84, no. 4, pp. 435–444, 1999. View at Google Scholar · View at Scopus
  11. World Medical Association, “World Medical Association Declaration of Helsinki. Ethical Principles for Medical Research Involving Human Subjects,” 2008, http://www.wma.net/en/30publications/10policies/b3/17c.pdf.
  12. T. W. Ridler and S. Calvard, “Picture thresholding using an iterative selection method,” IEEE Transactions on Systems, Man and Cybernetics, vol. 8, no. 8, pp. 630–632, 1978. View at Google Scholar · View at Scopus
  13. E. Lindner, “Die submikroskopische Morphologie des Herzmuskels,” Zeitschrift für Zellforschung und Mikroskopische Anatomie, vol. 45, no. 6, pp. 702–746, 1957. View at Publisher · View at Google Scholar · View at Scopus
  14. D. A. Nelson and E. S. Benson, “On the structural continuities of the transverse tubular system of rabbit and human myocardial cells,” The Journal of Cell Biology, vol. 16, no. 2, pp. 297–313, 1963. View at Google Scholar
  15. J. Amsellem, R. Delorme, C. Souchier, and C. Ojeda, “Transverse-axial tubular system in guinea pig ventricular cardiomyocyte: 3D reconstruction, quantification and its possible role in K+ accumulation-depletion phenomenon in single cells,” Biology of the Cell, vol. 85, no. 1, pp. 43–54, 1995. View at Publisher · View at Google Scholar · View at Scopus
  16. J. A. N. Fisher, B. M. Salzberg, and A. G. Yodh, “Near infrared two-photon excitation cross-sections of voltage-sensitive dyes,” Journal of Neuroscience Methods, vol. 148, no. 1, pp. 94–102, 2005. View at Publisher · View at Google Scholar · View at Scopus
  17. C. Soeller and M. B. Cannell, “Examination of the transverse tubular system in living cardiac rat myocytes by 2-photon microscopy and digital image-processing techniques,” Circulation Research, vol. 84, no. 3, pp. 266–275, 1999. View at Google Scholar · View at Scopus
  18. F. R. Heinzel, V. Bito, P. G. A. Volders, G. Antoons, K. Mubagwa, and K. R. Sipido, “Spatial and temporal inhomogeneities during Ca2+ release from the sarcoplasmic reticulum in pig ventricular myocytes,” Circulation Research, vol. 91, no. 11, pp. 1023–1030, 2002. View at Publisher · View at Google Scholar
  19. W. E. Louch, V. Bito, F. R. Heinzel et al., “Reduced synchrony of Ca2+ release with loss of T-tubules—a comparison to Ca2+ release in human failing cardiomyocytes,” Cardiovascular Research, vol. 62, no. 1, pp. 63–73, 2004. View at Publisher · View at Google Scholar · View at Scopus
  20. A. R. Lyon, K. T. MacLeod, Y. Zhang et al., “Loss of T-tubules and other changes to surface topography in ventricular myocytes from failing human and rat heart,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 16, pp. 6854–6859, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. N. Sperelakis and R. Rubio, “An orderly lattice of axial tubules which interconnect adjacent transverse tubules in guinea-pig ventricular myocardium,” Journal of Molecular and Cellular Cardiology, vol. 2, no. 3, pp. 211–220, 1971. View at Google Scholar · View at Scopus
  22. M. S. Forbes and E. E. van Niel, “Membrane systems of guinea-pig myocardiumml: ultrastructure and morphometric studies,” The Anatomical Record, vol. 222, no. 4, pp. 362–379, 1988. View at Publisher · View at Google Scholar
  23. J. G. Tidball, J. E. Cederdahl, and D. M. Bers, “Quantitative analysis of regional variability in the distribution of transverse tubules in rabbit myocardium,” Cell and Tissue Research, vol. 264, no. 2, pp. 293–298, 1991. View at Google Scholar · View at Scopus
  24. M. S. Forbes, L. A. Hawkey, and N. Sperelakis, “The transverse-axial tubular system (TATS) of mouse myocardiumml: its morphology in the developing and adult animal,” American Journal of Anatomy, vol. 170, no. 2, pp. 143–162, 1984. View at Google Scholar · View at Scopus
  25. E. H. Bossen, J. R. Sommer, and R. A. Waugh, “Comparative stereology of the mouse and finch left ventricle,” Tissue and Cell, vol. 10, no. 4, pp. 773–784, 1978. View at Publisher · View at Google Scholar · View at Scopus
  26. E. Page, L. P. McCallister, and B. Power, “Sterological measurements of cardiac ultrastructures implicated in excitation-contraction coupling,” Proceedings of the National Academy of Sciences of the United States of America, vol. 68, no. 7, pp. 1465–1466, 1971. View at Google Scholar · View at Scopus
  27. J. M. Stewart and E. Page, “Improved stereological techniques for studying myocardial cell growth: application to external sarcolemma, T system, and intercalated disks of rabbit and rat hearts,” Journal of Ultrastructure Research, vol. 65, no. 2, pp. 119–134, 1978. View at Google Scholar · View at Scopus
  28. F. Brette and C. Orchard, “T-tubule function in mammalian cardiac myocytes,” Circulation Research, vol. 92, no. 11, pp. 1182–1192, 2003. View at Publisher · View at Google Scholar · View at Scopus
  29. M. Kawai, M. Hussain, and C. H. Orchard, “Excitation-contraction coupling in rat ventricular myocytes after formamide-induced detubulation,” American Journal of Physiology, vol. 277, no. 2, pp. H603–H609, 1999. View at Google Scholar · View at Scopus
  30. P. Lipp, J. Hüser, L. Pott, and E. Niggli, “Spatially non-uniform Ca2+ signals induced by the reduction of transverse tubules in citrate-loaded guinea-pig ventricular myocytes in culture,” The Journal of Physiology, vol. 497, no. 3, pp. 589–597, 1996. View at Google Scholar · View at Scopus
  31. J. S. Mitcheson, J. C. Hancox, and A. J. Levi, “Action potentials, ion channel currents and transverse tubule density in adult rabbit ventricular myocytes maintained for 6 days in cell culture,” Pflügers Archiv European Journal of Physiology, vol. 431, no. 6, pp. 814–827, 1996. View at Publisher · View at Google Scholar · View at Scopus
  32. E. C. Keung, L. Toll, M. Ellis, and R. A. Jensen, “L-type cardiac calcium channels in doxorubicin cardiomyopathy in rats morphological, biochemical, and functional correlations,” Journal of Clinical Investigation, vol. 87, no. 6, pp. 2108–2113, 1991. View at Google Scholar · View at Scopus