Cell Signalling Pathways Leading to Novel Therapeutic Strategies in Cardiovascular DiseaseView this Special Issue
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Hannah J. Whittington, Girish G. Babu, Mihaela M. Mocanu, Derek M. Yellon, Derek J. Hausenloy, "The Diabetic Heart: Too Sweet for Its Own Good?", Cardiology Research and Practice, vol. 2012, Article ID 845698, 15 pages, 2012. https://doi.org/10.1155/2012/845698
The Diabetic Heart: Too Sweet for Its Own Good?
Diabetes mellitus is a major risk factor for ischemic heart disease (IHD). Patients with diabetes and IHD experience worse clinical outcomes, suggesting that the diabetic heart may be more susceptible to ischemia-reperfusion injury (IRI). In contrast, the animal data suggests that the diabetic heart may be either more, equally, or even less susceptible to IRI. The conflicting animal data may be due to the choice of diabetic and/or IRI animal model. Ischemic conditioning, a phenomenon in which the heart is protected against IRI by one or more brief nonlethal periods of ischemia and reperfusion, may provide a novel cardioprotective strategy for the diabetic heart. Whether the diabetic heart is amenable to ischemic conditioning remains to be determined using relevant animal models of IRI and/or diabetes. In this paper, we review the limitations of the current experimental models used to investigate IRI and cardioprotection in the diabetic heart.
Ischemic heart disease (IHD) is the leading cause of death and disability in the UK. Diabetes mellitus is a major risk factor for IHD patients with diabetes who are 2-3 times more likely to develop IHD . Diabetes mellitus (DM), in general, is a condition where the body cannot adequately control its level of glucose. There are two types of diabetes, type I diabetes and type II diabetes. Type I diabetes is characterized by the inability of the body to produce insulin; this is caused by cellular-mediated programming of the autoimmune system and subsequent destruction of pancreatic beta-cells, the cells responsible for insulin production. The prevalence of this form of diabetes is relatively low and usually starts early in life . Whereas type II diabetes is known as the insulin resistant form, insulin is produced however the target tissues become insensitive or resistant to the action of insulin thereby not utilizing blood glucose correctly and as a consequence increasing circulating blood glucose levels . This form accounts for a high percentage of all diabetes and is historically more common in older age groups; however the prevalence of type II diabetes in children and young adults is also increasing especially in westernized societies .
There are many features of the diabetic heart that can contribute to an increased susceptibility to IHD, these include diabetic cardiomyopathy and angiopathy . Diabetic cardiomyopathy is a disease that affects the myocardium in diabetic patients, causing structural changes which lead to abnormal functionality of the heart. These changes can eventually result in left ventricular hypertrophy (LVH) and diastolic/systolic dysfunction . Changes at the molecular level occur in the diabetic heart leading to this phenotype, including endothelial dysfunction, metabolic perturbations, and differences in cellular signaling, which are well described in an extensive review by Hayat et al., 2004 . Diabetic angiopathy causes vascular complications in chronic diabetic patients and can be classified into two types: microangiopathy and macroangiopathy . Diabetic microangiopathy is the term coined to describe the damage caused to small blood vessels and capillaries within the body as a consequence of chronic hyperglycemia. This damage can lead to a decreased supply of oxygen and vital substrates to the tissues, which can lead to adverse clinical outcomes such as retinopathy, nephropathy, neuropathy, and diabetic foot . Macroangiopathy as a consequence of diabetes mainly involves an accelerated form of atherosclerosis , another risk factor for CV disease. It affects the larger blood vessels within the body, where development and progression of atherosclerotic plaques lead to stenosis or occlusions, impairing blood flow . Diabetic complications, atherosclerosis, hypertension, and many other CV risk factors such as obesity can all interact and render the heart more susceptible to IHD .
The incidence of diabetes is increasing at an alarming rate throughout the world. Globally, the estimated prevalence of diabetes for 2010 was 285 million and is expected to affect 438 million people by 2030 . In the UK, there are 2.6 million people who have been diagnosed with diabetes (2009) which equates to a 4.1% average prevalence and it is estimated that 4 million people will be diabetic by 2025 .
The major consequence of IHD results from the detrimental effect of acute myocardial ischemia-reperfusion injury (IRI). IRI is a paradoxical event that occurs in the myocardium. Briefly, when blood flow is reduced due to myocardial ischemia, the best treatment strategy is to reestablish blood flow to the damaged area, however this return in blood flow causes damage . Patients with diabetes experience worse clinical outcomes in a number of clinical settings of acute IRI including acute myocardial infarction (MI) [14–16], coronary angioplasty , and cardiac bypass surgery [17–19]. This clinical data suggests that the diabetic heart may be more susceptible to acute IRI. In contrast, the animal data is inconclusive with experimental studies suggesting that the diabetic heart may be more, equally or even less susceptible to acute IRI . However, one major reason for the disparity between the clinical and animal data may be due to the choice of IRI and/or diabetic animal model used in the animal studies .
Given the worse clinical outcomes in diabetic patients with IHD, novel therapeutic strategies for protecting the diabetic heart against the detrimental effects of acute IRI are required to improve clinical outcomes in this patient group. Extensive research has investigated protecting the heart from IRI using conditioning strategies, either by mechanically activating cell survival pathways by ischemic preconditioning (IPC)  and remote ischemic conditioning (RIC)  or pharmacologically using cardioprotective agents . Ischemic conditioning is an endogenous phenomenon in which one or more brief cycles of nonlethal ischemia and reperfusion applied directly to the heart protects itself from a sustained lethal episode of acute IRI . Furthermore exciting research in the area of RIC, whereby an alternative organ to the heart, for example, the arm, can be made ischemic by one or more brief cycles of nonlethal ischemia and reperfusion using a blood pressure cuff, can limit the damage caused by a sustained ischemic insult  and this may provide a novel cardioprotective approach for the diabetic heart. These strategies are further described in Section 4. However, in order to translate ischemic conditioning into the clinical arena for the benefit of diabetic patients, it is important to first determine in animal studies whether the diabetic heart is amenable to cardioprotection elicited by ischemic conditioning. To achieve this requires the use of appropriate animal models of IRI and diabetes which also take into account other comorbidities such as age, dyslipidemia, and hypertension, factors which also impact on the ability to ischemic condition the heart .
The aim of this paper will be to highlight the limitations of currently used animal models of IRI and diabetes as a potential explanation for the disparity that exists between clinical and experimental data, with regard to the susceptibility of the diabetic heart to IRI and endogenous cardioprotection.
2. Animal Models of Diabetes
Animal models used to investigate diabetes have been created using a variety of different methods such as the administration of drugs toxic to the pancreas, modified diets, inbreeding of spontaneous mutations, or genetic engineering . This has resulted in the availability of many different diabetic animal models . In addition, the diabetic status induced by these methods needs to be assessed with caution as none of the models accurately and completely reflect the human pathology of diabetes.
For example, type I diabetes is characterized by insulin deficiency as a consequence of autoimmune destruction of pancreatic β cells in the islets of Langerhans . This disease is mimicked by administration of streptozotocin (STZ). However, this is an alkylating agent based nitrosourea derivative , which interferes with numerous cellular processes such as glucose transport, glucokinase function and can also induce DNA strand breaks . This toxic compound can be given either as a single high dose to induce diabetes but this is associated with high mortality. Therefore, it is more common to use a series of low doses to induce diabetes in rodents . Alloxan is a toxic glucose analogue, which preferentially accumulates in beta cells of the pancreas, causing excessive production of hydroxyl free radicals and destruction of the beta-cells hence mimicking type I diabetes . In essence, the primary etiopathology caused by these experimental approaches has little autoimmune component and does not truly reflect the pathogenesis leading to type I diabetes in the human . Therefore, it could be argued that the translation of findings from this particular animal model of type I diabetes to the clinical setting may be problematic.
Type II diabetes in humans is a metabolic disorder and normally arises during adulthood . A good animal model of type II diabetes is the Goto-Kakizaki (GK) rat, which originates from the inbreeding of Wistar rats that exhibited hyperglycemia. The GK rat spontaneously becomes diabetic early in life, showing glucose insensitivity in their pancreatic beta cells  with the diabetic status increasing with age . The Otsuka Long Evans Tokushima Fatty (OLETF) rat originates from the inbreeding of Long-Evans rats which exhibit glucose intolerance ; these rats are mildly obese and the diabetic phenotype is more dramatic in males. Both the Zucker diabetic fatty (ZDF) rat  and the db/db mice  are models that express other comorbidities such as obesity and dyslipidemia as well as glucose intolerance . For a thorough review into animal models in diabetes please refer to .
3. The Susceptibility of the Diabetic Heart to Acute IRI
Despite the clinical data suggesting that the diabetic heart is more susceptible to acute IRI, the animal data has been conflicting with experimental studies showing more, equal, or less sensitivity to acute IRI. The reasons for this disparity between the animal and clinical data were the subject of a review in 1997 by Paulson, who concluded that the sensitivity of the diabetic heart to acute IRI was dependent on the animal models and conditions used. The diabetic heart was shown to be less sensitive to acute IRI in studies which (i) used a short duration of diabetes (<6 weeks); (ii) used glucose as the only substrate; (iii) used a no-flow IRI protocol, whereby global ischemia is initiated by total interruption of the perfusate to the heart. Whereas, if diabetes was more prolonged and severe, fatty acids were present in the perfusate and a low-flow IRI protocol was used that is, experimental protocols which reflect the clinical scenario better, the diabetic heart was found to be more sensitive to IRI . We have reviewed the literature since 1997, and the same pattern emerges (see Tables 1, 2, and 3). However, to this we need to add another complicating factor, the choice of IRI model and the lack of other comorbidities such as age, dyslipidemia, and hypertension, factors which are critical when investigating cardioprotection.
Numerous endogenous factors contribute to myocardial IRI. These include production of reactive oxygen species (ROS), changes in the intracellular calcium and pH, triggering of inflammatory mechanisms; all of which interact with each other to mediate opening of the mitochondrial permeability transition pore (PTP), leading to eventual cardiomyocyte death (Figure 1) . Interestingly, the pathological changes already occurring in diabetic cardiomyopathy include increased release of ROS , abnormal handling of calcium, and increased release of inflammatory mediators ; this experimental information alongside the evidence that diabetic patients have a worse prognosis following myocardial infarction , strongly suggest that the diabetic heart should be more vulnerable to damage. However, a plethora of experimental and clinical studies demonstrated a variety of outcomes, which could be highly dependent on the animal model, experimental protocol, and severity of diabetes. In Figure 2, we attempt to summarize the possible mechanisms that make the diabetic heart more or less susceptible to infarction following ischemia reperfusion.
3.1. Animal Models of Type I Diabetes
A vast amount of research has been performed in order to assess the sensitivity to myocardial ischemic damage in models of type I diabetes. In vivo [53, 56] and ex vivo  investigations using a STZ-rat model demonstrated that acute diabetes (1–4 weeks of STZ treatment) resulted in less susceptibility to acute IRI. However, if STZ treatment was increased to longer than 6 weeks, the sensitivity to acute IRI was increased. Ma et al.  found increased phosphorylation of the prosurvival kinase Akt and decreased levels of caspase-3, vascular endothelial growth factor (VEGF), and nitric oxide (NO) following 2 weeks STZ induction but the opposite finding following 6 weeks treatment, supporting the loss of the cardioprotective state after 6 weeks . Nawata et al. (2002)  also found that 4 weeks of STZ-induced diabetes reduced sensitivity to acute IRI in the Langendorff-perfused isolated heart model . This phenomenon of protection in the acute diabetic setting was also present in alloxan-induced diabetes in Yucatan pigs . Following 1 hour of regional coronary artery occlusion and reperfusion the in vivo myocardial infarct size was smaller compared to control and this was accompanied by an increased expression of cell survival proteins. Of note, global left ventricular function was worse in diabetes; however function within the area at risk was better . In a cardiomyocyte model of diabetes, cells were incubated for 3 days with either 5 mM or 25 mM glucose in the medium. High-glucose treatment was protective against simulated IRI. The high-glucose treatment caused a reduction in necrosis, apoptosis, and calcium content, whereas the antiapoptotic protein bcl-2 increased and proapoptotic bad was shifted to its inactive state  in the presence of 25 mM glucose.
In contrast to this data, showing that “acute” or short-term induced type I diabetes is cardioprotective compared to the longer-term induction, Hadour et al. (1998)  induced diabetes in rabbits for 8 weeks using alloxan, then subjected the hearts to in vivo 30 minute myocardial ischemia and 3 hours reperfusion and saw a reduction in MI size compared to control nondiabetic rabbits. Conversely, in nondiabetic rabbits infused with high glucose throughout myocardial ischemia and reperfusion to mimic acute diabetes no difference in infarction was seen compared to controls. They suggested that the presence of type I diabetes in the rabbit induced a chronic and metabolic cardioprotective state in the heart . Ebel et al.  showed in the same model of alloxan-induced diabetes in rabbits that 6-weeks duration of diabetes had no influence on the vulnerability to IRI compared to controls, further supporting the theory of a chronic (greater than 6 weeks) protected state in this model . Chronic administration of STZ for 12 weeks in rats also showed a decreased susceptibility to MI . Diabetic hearts had greater left ventricular function and the incidence of ventricular fibrillation and creatine kinase (CK) release were decreased. This was accompanied by a persistent translocation of protein kinase C-ε (PKC-ε), a modulator of the mitochondrial permeability transition pore, during ischemia but only in the diabetic hearts. Following these results, the authors suggested that in STZ-induced diabetes PKC-ε plays a crucial role in the susceptibility to MI . However, this investigation was performed using a Langendorff-perfused model of no flow ischemia and contained no added substrates. It would be interesting to assess translocation properties of PKC-epsilon in the in vivo setting.
Other investigators have also shown that acute initiation of type I diabetes does not influence the susceptibility to MI. Both alloxan and STZ-treated dogs, although at a lower dose to previously mentioned investigations, reported no difference in MI size following IRI in vivo .
However, in other studies, type I diabetes has been shown to render the heart more susceptible to IRI. It has been suggested that hyperglycemia has a negative impact on endogenous cardioprotective signaling. Kersten et al.  showed that hyperglycemia in dogs, induced either acutely by 15% dextrose prior to IRI in vivo, or in chemically induced diabetes (3 weeks STZ), increased MI size. Interestingly, the studies mentioned above suggested that “acute” diabetes would render the heart less susceptible to diabetes. However, those studies were in rodent models of diabetes and had a shorter duration of IRI. Ebel et al.  recorded a greater susceptibility to MI in their rabbit model following an infused solution of 50% dextrose 30 minutes prior to ischemia in vivo to elicit a hyperglycemic state of 600 mg/dL . Su et al.  used an infused glucose model to elicit hyperglycemia in rats, this also rendered the heart more susceptible to MI of note the reperfusion time following ischemia in this study was notably longer compared to other rodent models . With regard to the possible mechanism, hyperglycemia in rat cardiomyocytes was shown to promote p53-dependent activation of apoptosis .
Marfella et al.  also examined the effect of hyperglycemia and STZ-induced diabetes on MI size in rats, both in vivo and ex vivo. Both hyperglycemic conditions caused an increase in MI size, together with a decreased transactivation of the hypoxic inducible factor HIF-1α . The diabetic induction in this model was 9 days prior to the ischemic episode, although an increased dose of STZ (70 mg/kg) was used. HIF-1α controls the upregulation of vascular endothelial growth factor (VEGF) in response to hypoxia, initiating neovascularization following ischemia. If this axis is impaired, as seems the case in diabetes, inadequate revascularization will occur resulting is potential worse outcomes for the diabetic patient .
3.2. Animal Models of Type II Diabetes
In 2004, Kristiansen et al.  were the first to investigate the susceptibility to acute IRI and ischemic conditioning (IPC) in a model of type II diabetes. Two distinct models: the GK rat and ZDF rat showed in Langendorff-perfused isolated hearts (with no added substrates), that MI size was smaller than their respective nondiabetic controls. Interestingly, even though these hearts appeared to be more resistant to ischemic damage, they were not amenable to protection by IPC . Desrois et al. performed two separate studies also using the ex vivo Langendorff preparation in the GK rat in 2004  and 2010 . In 2004, they compared gender difference within diabetes and the effect this has on the susceptibility to MI. The preparation involved perfusing the hearts with Krebs-Hensleit buffer with no added substrates. They found that female hearts had larger MI size than the male GKs and that no significant difference was noted between male hearts of the diabetic and nondiabetic controls . Interestingly, their study in 2010 again utilized the Langendorff technique, and showed that the male GK heart was more susceptible to MI than the control heart . The latter investigation included additional fatty acid substrates in the perfusate, similar to those likely to be found in the in vivo scenario. This may suggest that in the setting of type II diabetes, substrates found in the blood could play an important role in cell damage. Studies in db/db mice in vivo support this idea; Lefer et al. (2001)  and Jones et al. (1999)  both saw a diabetes-associated increase in MI size.
Other studies show a similar result to Kristiansen et al’s study in 2004 . Tsang et al. (2005) using the isolated heart model, with no added substrates, however with a shorter ischemic time, showed the heart of the GK rat to be less susceptible to infarction than the Wistar rat control . Supporting these findings, cardiomyocytes isolated from GK rats were less susceptible to mPTP opening in response to calcium, achieved by adding soluble Ca2+ to a phosphate-containing medium. This was accompanied by a larger calcium accumulation, leading to decreased opening of the mitochondrial pore and reduced cardiomyocyte death . Controversially, two recent investigations that have been performed in vivo [64, 65] in the GK rat showed no difference in the susceptibility to infarct compared to control rats following 30 or 35 minutes ischemia and 2-hour reperfusion.
Some blood components, such as platelets and neutrophils, have been suggested to play a role as mediators of cell damage in ischemia and reperfusion . This is another confounding factor when comparing data from cell, in vitro and in vivo experiments. The sensitivity of the type II diabetic heart in in vivo settings has had limited study compared to type I diabetes, therefore more work is required to clarify these initial findings.
4. Cardioprotection in the Diabetic Heart
4.1. Animal Models of IRI
The heart, as in any living tissue, has endogenous protective mechanisms which, when activated, render it resistant to IRI, in other words the heart can be “conditioned” . Initial studies by Murry et al. (1986) , showed that short bursts of ischemia followed by reperfusion prior to a prolonged insult of ischemia and reperfusion, were associated with a reduction in MI size by 75%. This was termed “ischemic preconditioning” (IPC) . The phenomenon of IPC is highly reproducible in all species . Over the years, the phenomenon of IPC has evolved to include pharmacological conditioning , which involves targeting cellular mechanisms involved in IRI or promoting those involved in IPC. Also, short bursts of ischemia and reperfusion following an ischemic insult can reduce MI size and this term was coined ischemic postconditioning (IPost) . More recently, remote ischemic conditioning has been discovered, whereby an organ or tissue remote from the heart is conditioned and reduces infarct size .
IPC reduces lethal cell injury in the ischemic myocardium ; how this phenomenon works has been extensively studied and is summarized in Figure 3. Briefly, IPC causes the release of G-protein-coupled receptor (GPCR) agonists which bind to the receptor and activate numerous signaling pathways. Phosphatidylinositol-3-kinase (PI3K) activation can lead to activation of a number of downstream molecules such as Akt, protein kinase C (PKC), extracellular regulated kinase (ERK), nitric oxide synthase (NOS), and inactivation of glycogen synthase kinase-3β (GSK-3β). These converge to activate the mitochondrial ATP-dependent potassium channel (KATP), closing the mitochondrial permeable transition pore (mPTP) resulting in protection from IRI . Before IPC can be applied in the clinical setting it is important to determine whether diabetic heart is amenable to this endogenous cardioprotective strategy. The animal data suggests that the diabetic heart is resistant to ischemic conditioning such that the IPC stimulus needs to be increased to induce cardioprotection .
Bouchard et al. (1998)  showed that in a STZ rat model of diabetes, an IPC stimulus consisting of 1 cycle of 5-minute ischemia followed by 10-minute reperfusion (IPC-1) was not sufficient to preserve the vasodilatory response to 5-HT suggesting an impairment of endothelial cells in the diabetic heart and consequently the inability to generate and release enough NO. However, when the IPC stimulus was increased to 3 cycles of 5-minute ischemia followed by 5-minute reperfusion (IPC-3) the vasodilatory response to 5-HT was present. Furthermore, in the same investigation, 30-minute pretreatment with adenosine, an important mediator in IPC , mimicked the cardioprotective outcome of IPC-3, whereas 15-minute pretreatment was ineffective . These findings suggested that signaling pathways activated by adenosine either endogenously or exogenously may be hindered in diabetic coronary vessels, but have the potential to be recovered by an increased stimuli. The KATP channel is involved in the mechanism of protection by IPC  and adenosine ; studies in diabetic animals have reported reduced sensitivity to KATP channel activators  which could correlate with the need for an increased stimulus to achieve KATP channel activation, subsequent mPTP inhibition, and reduction in apoptosis initiation hence reducing myocardial cell death . Another study using alloxan-/STZ-induced diabetes in dogs also supported the role of dysfunctional KATP channel in diabetes .
Other important endogenous mechanisms involved in protection by IPC is the reperfusion injury salvage kinase pathway (RISK) , with its main constituent the PI3K-Akt axis. Tsang et al. (2005)  found that to protect the myocardium in the Goto-Kakizaki rat model of type II diabetes, an increased IPC stimulus was also required. Similar to the previous study, 1 cycle of 5-minute ischemia followed by 10-minute reperfusion was insufficient to protect the heart; however 3 cycles of this protocol reduced infarct volume considerably. They investigated the level of phosphorylated Akt (Akt-P) within the myocardium and found that in the diabetic heart, an increased IPC stimulus is required to achieve an essential level of Akt phosphorylation necessary to mediate cardioprotection , thereby highlighting a diabetes-associated impairment in PI3K-Akt signaling. Increasing the stimulus of IPC by increasing cycle number appears important in diabetes; however, the duration of the pre-ischemia/reperfusion protocol may also play a vital role. Four cycles of 2-minute ischemia followed by 3-minute reperfusion did not elicit protection in two separate models of diabetes , compared to 3 cycles of 5-minute ischemia and 10-minute reperfusion used by Tsang et al.  in the same animal and experimental setting.
A recent study by Hotta et al. (2010)  using the OLETF rat model of type II diabetes showed that pretreatment with either opioid agonist  or erythropoietin (EPO) , known to mediate IPC signaling through Janus kinase (Jak-2), failed to elicit cardioprotection in vivo due to insufficient phosphorylation of Jak-2 and Akt, and like others, also found an elevated level of calcineurin activity in diabetic hearts . Interestingly, following 2-week treatment with either valsartan or losartan, Angiotensin II receptor type 1 (AT1) receptor blockers, Jak-PI3K-Akt signaling, and hence cardioprotection subsequent to EPO administration were restored . Huisamen et al. (2011) supported the finding that AT1 antagonism can lead to cardioprotection in their ex vivo rat model of insulin resistance, diet-induced obesity (DIO) rat .
In contrast, Ghaboura et al. (2011), examined EPO-induced IPost cardioprotection in two rat models of type I diabetes; STZ-induced and high-fat-diet (HFD-) induced insulin resistance syndrome. They found that EPO administered at the onset of reperfusion did not increase phosphorylation of Akt, ERK, or GSK-3β and hence did not elicit a cardioprotective effect in the hearts isolated from STZ rats; interestingly, HFD rat hearts were protected following EPO. Administration of a GSK-3β antagonist, a kinase which is downstream of the PI3K-Akt axis, given prior to ischemia and continued throughout reperfusion had an infarct limiting effect . In support of the idea that inhibiting GSK-3β in diabetic hearts as a direct cardioprotective strategy, Gross et al. (2007)  showed that diabetes had a detrimental effect on morphine-induced protection. STZ-induced diabetes caused alterations in pathways upstream of GSK-3β, such as PI3K, Jak/STAT, and MAPK pathways, limiting the cardioprotective effects of morphine administered at reperfusion. Similarly, downstream antagonism of GSK-3β was cardioprotective . The direct inhibition of GSK-3β was also proved cardioprotective in a rat model of type II diabetes . In this study, another diabetic-associated myocardial phenotype was reported; they found that increased endoplasmic reticulum stress, caused alterations in PI3K-Akt, ERK, and GSK-3β signaling motifs, and hence dysregulation of the mPTP leading to the absence of cardioprotection by EPO .
The idea that the diabetic heart is still amenable to protection but has an increased threshold for the necessary activation of pro survival kinases is an interesting prospect. Investigations by Tsang et al.  and Bouchard et al.  clearly demonstrated this; however, in the study by Ghaboura et al.  increasing the dose of EPO did not restore cardioprotective effects. Of course, these studies are performed in a variety of models of diabetes, with variable levels of diabetic severity. Tsang et al.  used a model with moderate hyperglycemia, however both Bouchard et al.  and Ghaboura et al.  induced diabetes by injections of STZ at 55 mg/kg and 65 mg/kg, respectively. Therefore, speculation that the ability to protect the diabetic heart can be restored by an increased stimulus in moderate hyperglycemia but not severe hyperglycemia could be flawed and needs further investigation.
All of the mentioned studies have furthered the understanding of the mechanisms that could render the diabetic heart ineffective to cardioprotective strategies, and how we could potentially overcome these hurdles. Though a variety of animal models were used, including in vivo and ex vivo experimental protocols, direct targeting of GSK-3β in the diabetic heart may provide an alternative solution to prevent ischemia reperfusion injury . However, like any therapy, a targeted approach would have to be considered to limit any potential negative effects .
4.2. Experimental Studies Using Human Heart Tissue
As part of the translational process, our laboratory have established since 1995 an isolated human atrial muscle preparation of simulated IRI . This preparation has become a popular tool in investigating the response of human tissue in cardiovascular diseases. In brief, samples of right atrial appendage are obtained from the right atrial cannula insertion site in patients undergoing cardiac bypass during coronary artery bypass graft surgery . From the right atrial appendage, trabeculae are isolated and subjected to simulated IRI and the recovery of basal function assessed following IRI.
Numerous studies have been performed investigating the mechanisms of preconditioning in the normoglycemic human myocardium ; however a limited number of studies also investigated this phenomena in the diabetic human heart. Using a variation of this model in which the right atrial appendage is sliced and subjected to simulated IRI, Ghosh et al. (2001) , showed the failure of IPC to protect the diabetic human myocardium with 1 cycle of 5-minute ischemia and 5-minute reperfusion reflecting what was already demonstrated in animal investigations, that is, an increased threshold for cardioprotection in the hyperglycemic tissue. As previously mentioned KATP channels play a role in IPC. Diazoxide, a KATP opener, did not mimic IPC in diabetic tissues suggesting a dysfunctional mitochondrial KATP channel . This result was supported by the finding that the ATP-sensitive potassium channels are altered in ventricular myocytes from diabetic rats . Of interest, this group did not try increasing the IPC stimulus to see if the human muscle mirrored the finding from Tsang and colleagues . Hassouna et al. (2006) also suggested that the dysfunctional mitochondrial KATP channel exists in diabetics, causing impaired depolarization and superoxide production, resulting in an inability to respond to IPC . Increasing the IPC stimulus to 3 cycles in this experimental model did not restore the protection , conflicting with the finding of Tsang et al. . However, Sivaraman et al. (2010)  showed that increasing the duration of ischemic period of IPC from 4 minutes to 7 minutes followed by reoxygenation in human diabetic atrial tissue restored cardioprotection and similarly was related to a downregulation of PI3K-Akt axis in diabetic tissue .
In a recent study performed by Linares-Palomino et al. specific inhibitors of Akt were utilized to delineate its specific role in ischemic pre conditioning. They showed that blockade of Akt caused a significant reduction in cell death, similar to the degree of protection elicited by either IPC or PI3K inhibition and this was evident in both rat and human tissue. Interestingly, they showed Akt to be downstream of mitochondrial KATP channel but upstream of p38 MAPK .
This data reinforces the fact that cardioprotection in the diabetic heart is a complex and delicate phenomenon; the knowledge gained from the extensive research throughout many models has highlighted some potential reasons why it is more difficult to protect the diabetic heart, reasons which are summarized in Figure 4.
Proof-of-concept clinical studies are now required to determine whether the human diabetic heart is amenable to cardioprotection elicited by ischemic conditioning. Preliminary unpublished data from our research group suggests that the diabetic patient may not be amenable to cardioprotection elicited by remote ischemic conditioning (RIC). There was no difference in perioperative myocardial IRI (72 hour area under the curve serum Troponin T) in diabetic patients randomized to receive a standard RIC stimulus (three 5-minute inflations and deflations of a blood pressure cuff on the upper arm) prior to CABG surgery (Babu et al. unpublished), a RIC protocol which has been previously reported to protect nondiabetic patients .
5. Limitation of Translation and Future Directions
The obvious limitation of translating research from bench to bedside is that many of the animal models we use do not adequately reproduce the clinical setting or fully represent the disease pathology and manifestation in patients. In addition, the vast majority of studies are conducted on inbred animals with very little genetic variability; they are fed ad libitum with scientific diets and are kept in optimal living conditions of light and temperature. This is far from the complexity of patients who possess a large genetic pool, undergo the stresses of day to day working life, some eating high-fat diets and undertaking limited exercise. In addition, the majority of people in westernized societies take a plethora of medications.
There is an increasing amount of research now being conducted in animals with comorbidities to promote successful translation of the research field of cardioprotection. The design of the preclinical model, either in human tissue or animal models, is critical to harness the huge potential of IPC as a strategy to reduce ischemic damage. Preliminary unpublished data from our research group, utilizing aging, diabetic rats suggests an age-associated increase to the threshold of IPC and susceptibility to infarction in models of type II diabetes (Whittington et al. unpublished). These results could highlight a possible discrepancy from original IPC studies performed in young healthy animals and could indicate why the findings were not transferable to the clinic.
It is well established that diabetic patients with IHD experience worse clinical outcomes yet the animal data is conflicting. The choice of the diabetic and IRI animal model may in part explain this discrepancy. The development of novel cardioprotective strategies for protecting the diabetic heart and improving clinical outcomes in diabetic patients with IHD will be dependent on using relevant animal models of IRI and diabetes which also take into account other comorbidities such as age, dyslipidaemia, and hypertension. Therefore, by carefully selecting and optimizing clinically relevant animal models of IRI and diabetes, we may be able to better translate findings made at the “bench” to patients’ “bedside”.
Conflict of Interests
There is no conflict of interests to declare.
The authors thank the British Heart Foundation (Program Grant RG/03/007 and FS/10/039/28270) for ongoing funding and support. This work was undertaken at University College London Hospital/University College London (UCLH/UCL) who received a proportion of funding from the Department of Health’s National Institute of Health Research (NIHR) Biomedical Research Centres funding scheme.
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