Neutralization by Insulin of the Hypertensive Effect of Dermcidin Isoform 2: An Environmentally Induced Diabetogenic and Hypertensive Protein

Figure 5

The opposing effect of dermcidin and insulin on the synthesis of (r)-cortexin. Typically kidney cortex cells homogenate in Kreb’s buffer, pH 7.0, was prepared as described before [11]. The incubation of the suspension with 0.2 μM dermcidin in the presence and absence of different amounts of insulin was carried out as described in Section 2. The in vitro translation of the (r)-cortexin mRNA was done as described in Section 2 and the (r)-cortexin synthesis was quantitated by ELISA. Solid bar represents the control experiment using only kidney cortex cells. The patterned bar represents incubation of kidney cortex cells treated with 0.2 μM dermcidin. Gray bars indicate the incubation of kidney cortex cells treated with 0.2 μM dermcidin in the presence of different amounts of insulin (μunits/mL). The significance of the results obtained between the different groups (A–G) has been shown in the figure. The results shown are mean ± SD of 10 different experiments using 5 different goat kidney cortex cells preparation, each in triplicate.