RyR2 QQ2958 Genotype and Risk of Malignant Ventricular Arrhythmias

Table 1

Primers sequences, annealing temperatures, and fragment size.

Gene	SNP	Allele	Primer sequence 5′→3′	Fragment size (bp)
RyR2	Q2958R (A>G)	A	Upper: GGAGAACATTTCCCTTATGACCA	476	60°C
			Lower: GCAGGACTAAGGTCCCACAA
		G	Upper: GAGAACATTTCCCTTATGACCG	476	60°C
			Lower: GCAGGACTAAGGTCCCACAA
HRC	S96A (G>T)	G	Upper: AAGGAGGATGAAGATGCCG	347	62°C
			Lower: TCCTCTTCCTCCTCCTCCTC
		T	Upper: AAAAGGAGGATGAAGATGCCT	347	62°C
			Lower: TCCTCTTCCTCCTCCTCCTC

Bold letters in primer sequences highlight the allele-specific nucleotide, while underlined letters highlight mismatch nucleotide.