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Cardiology Research and Practice
Volume 2016 (2016), Article ID 3942605, 8 pages
http://dx.doi.org/10.1155/2016/3942605
Research Article

Progenitor Hematopoietic Cells Implantation Improves Functional Capacity of End Stage Coronary Artery Disease Patients with Advanced Heart Failure

1Department of Cardiology and Vascular Medicine, Faculty of Medicine, University of Indonesia and National Cardiovascular Center Harapan Kita, Jakarta 11420, Indonesia
2Stem Cell and Cancer Institute, Jakarta 13210, Indonesia

Received 19 January 2016; Accepted 20 March 2016

Academic Editor: Terrence D. Ruddy

Copyright © 2016 Yoga Yuniadi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Proangiogenic Hematopoietic Cells (PHC) which comprise diverse mixture of cell types are able to secrete proangiogenic factors and interesting candidate for cell therapy. The aim of this study was to seek for benefit in implantation of PHC on functional improvement in end stage coronary artery disease patients with advanced heart failure. Methods. Patients with symptomatic heart failure despite guideline directed medical therapy and LVEF less than 35% were included. Peripheral blood mononuclear cells were isolated, cultivated for 5 days, and then harvested. Flow cytometry and cell surface markers were used to characterize PHC. The PHC were delivered retrogradely via sinus coronarius. Echocardiography, myocardial perfusion, and clinical and functional data were analyzed up to 1-year observation. Results. Of 30 patients ( yo) preimplant NT proBNP level is  pmol/L. Harvested cells characterized with CD133, CD34, CD45, and KDR showed , , , and %, respectively. LVEF was improved ( versus , ) during short and long term observation. Myocardial perfusion significantly improved 6 months after treatment. NYHA Class and six-minute walk test are improved during short term and long term follow-up. Conclusion. Expanded peripheral blood PHC implantation using retrograde delivery approach improved LV systolic function, myocardial perfusion, and functional capacity.