Table of Contents
Developmental Biology Journal
Volume 2014, Article ID 432043, 12 pages
http://dx.doi.org/10.1155/2014/432043
Research Article

Cooperation of Nectin-1 and Nectin-3 Is Required for Maintenance of Epidermal Stratification and Proper Hair Shaft Formation in the Mouse

1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cyo, Shinjuku-ku, Tokyo 162-8666, Japan
2Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650-0047, Japan
3Institute of Biotechnology, University of Helsinki, P.O. Box 56, 00014 Helsinki, Finland

Received 13 February 2014; Accepted 1 June 2014; Published 30 June 2014

Academic Editor: De-Li Shi

Copyright © 2014 Toshiyuki Yoshida et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1. Expression patterns of nectin-1 and nectin-3 mRNA in embryonic day 17 skin. Nectin-1 is expressed in the epidermis and hair follicle while nectin-3 is not expressed in the epidermis and hair follicle at embryonic day 17.

Figure S2. Macroscopic pictures of ventral skin of control and nectin-1-/-; nectin-3+/- mice. The ventral skin of nectin-1-/-; nectin-3+/- mice exhibited reduced hair follicle than that of control mice.

Figure S3. Triple staining with antibodies against p63 and K14 and with DAPI for the nuclei in nectin-1-/-; nectin-3+/- epidermis. The cells that exhibited reduced expression of K14 are negative for p63 in nectin-1-/-; nectin-3+/- epidermis.

Figure S4. Immunohistochemical analysis of p63 and Ki67 in dorsal hair follicle of control and nectin-1-/-; nectin-3+/- skin. The remaining expression of Ki67 and p63 are observed in the nectin-1-/-; nectin-3+/-hair follicles.

Figure S5. The histological and immunohistochemical analysis of embryonic skin of control and compound mutant at embryonic day 14. The skin of nectin-1-/-; nectin-3-/- mice is comparable to that of control mice.

Figure S6. Macroscopic pictures of control and nectin-1-/-; nectin-3+/- embryo at embryocnic day 17. The gross appearance of nectin-1-/-; nectin-3+/- is comparable to that of control mice at embryonic day 17.

  1. Supplementary Material