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Disease Markers
Volume 12 (1994), Issue 2, Pages 147-165
http://dx.doi.org/10.1155/1994/563543

Cytokine Receptor Expression in Human Lymphoid Tissue: Analysis by Fluorescence Microscopy

Heddy Zola,1 Jodie Ridings,1 Helen Weedon,1 Michael Fusco,1 Roger W. Byard,1 and Peter J. Macardle3

1Child Health Research Institue, Adelaide, Australia
2Department of Paediatrics, Women's and Children's Hosptial, Adelaide, Australia
3Flinders Medical Center, Adelaide, Australia

Received 20 May 1994

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A highl y-sen sitile tlourescence method, capable of detecting cytokine receptors present at low concentrations (around I DO molecules per cell) by flow cytometry, was adapted for use on tissue sections. This method was used to examine the expression of several cytokine receptors in lymphoid ti ss ues. lL-2 receptors were distributed broadly, with higher concentrations in T cell areas. lL-1 receptor Type I was detected in T cell areas and in the follicular mantle, and was strongly expressed on vasc ular endothelium. IL-6 receptor was found at very low concentration, both within and outside germinal centres. The gp 130 molecule, which is involved in the functional receptor complex for IL-6 and several other cytokines, was present at higher concentrations, particularly in the germinal centre. Analysis of receptor expression in secondary lymphoid tissue provides evidence bearing on the physiological roles of cytokines, as these tissues contain cells at various stages of physiological activation located in well-defined functional zones.