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Disease Markers
Volume 17, Issue 4, Pages 205-216
http://dx.doi.org/10.1155/2001/202814

Proteome Analysis—A Novel Approach to Understand the Pathogenesis of Type 1 Diabetes Mellitus

Allan E. Karlsen, Thomas Sparre, Karin Nielsen, Jørn Nerup, and Flemming Pociot

Steno Diabetes Center, Gentofte, Denmark

Received 19 November 2001; Accepted 19 November 2001

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Type 1 (insulin-dependent) diabetes mellitus (T1DM) is associated with a specific destruction of the insulin-producing beta-cells in the islets of Langerhans. Several factors, e.g. genetic, environmental and immunologial, may be involved in the etiology and pathogenesis of T1DM. Autoreactive Tand B-lymphocytes, together with macrophages infiltrate the islets during the pathogenesis, releasing a mixture of cytokines, demonstrated to be specifically toxic to the beta-cells within the islets. Our goal is to understand the molecular mechanisms responsible for the beta-cell specific toxicity enabling us to design novel intervention strategies in T1DM. The proteome approach allows us to get a detailed picture of the beta-cell proteins, which change expression level or are post-translationally modified in different in vitro and in vivo models of T1DM-associated beta-cell destruction. Combining the information obtained from this extended proteome approach, with that of genetic-, transcriptome- and candidategene approaches, we believe that it is possible to reach this goal.