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Disease Markers
Volume 17, Issue 3, Pages 173-178

Potential Cellular Signatures of Viral Infections in Human Hematopoietic Cells

J. Mikovits,1 F. Ruscetti,2 W. Zhu,1 R. Bagni,3 D. Dorjsuren,1 and R. Shoemaker4

1Laboratory of Antiviral Drug Mechanisms, Frederick, MD, USA
2Laboratory of Leukocyte Biology, DBS, National Cancer Institute-Frederick Cancer Research Center, Frederick, MD 21702-1201, USA
3Intramural Research Support Program SAIC Frederick, Frederick, MD, USA
4Screening Technologies Branch, Developmental Therapeutics Program, DCTD, Frederick, MD, USA

Received 31 October 2001; Accepted 31 October 2001

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Expression profiling of cellular genes was performed using a 10,000 cDNA human gene array in order to identify expression changes following chronic infection of human hematopoietic cells with Kapsosi’s Sarcoma -associated Virus (KSHV) also known as Human Herpesvirus 8 (HHV8) and Human T cell leukemia virus-1 (HTLV-1). We performed cell-free {\it in vitro} infection of primary bone marrow derived CD34+ cells using semi-purified HHV8 and a mature IL-2 dependent T cell line, KIT 225, using highly concentrated viral stocks prepared from an infectious molecular clone of HTLV-1. Thirty days post infection, mRNA was isolated from infected cultures and uninfected controls and submitted for microarray analysis. More than 400 genes were differentially expressed more than two-fold following HHV8 infection of primary bone marrow derived CD34+ cells. Of these 400, interferon regulatory factor 4 (IRF4), cyclin B2, TBP-associated factor, eukaryotic elongation factor and pim 2 were up-regulated more than 3.5 fold. In contrast, less than 100 genes were differentially expressed more than two-fold following chronic infection of a mature T cell line with HTLV-1. Of these, only cdc7 was up-regulated more than 3.5 fold. These data may provide insight into cellular signatures of infection useful for diagnosis of infection as well as potential targets for therapeutic intervention.