Disease Markers

Disease Markers / 2002 / Article

Open Access

Volume 18 |Article ID 462465 | https://doi.org/10.1155/2002/462465

Catherine Campbell, Suzanne D. Vernon, Kevin L. Karem, Rosane Nisenbaum, Elizabeth R. Unger, "Assessment of Normal Variability in Peripheral Blood Gene Expression", Disease Markers, vol. 18, Article ID 462465, 6 pages, 2002. https://doi.org/10.1155/2002/462465

Assessment of Normal Variability in Peripheral Blood Gene Expression

Received12 Feb 2003
Accepted12 Feb 2003


Peripheral blood is representative of many systemic processes and is an ideal sample for expression profiling of diseases that have no known or accessible lesion. Peripheral blood is a complex mixture of cell types and some differences in peripheral blood gene expression may reflect the timing of sample collection rather than an underlying disease process. For this reason, it is important to assess study design factors that may cause variability in gene expression not related to what is being analyzed. Variation in the gene expression of circulating peripheral blood mononuclear cells (PBMCs) from three healthy volunteers sampled three times one day each week for one month was examined for 1,176 genes printed on filter arrays. Less than 1% of the genes showed any variation in expression that was related to the time of collection, and none of the changes were noted in more than one individual. These results suggest that observed variation was due to experimental variability.

Copyright © 2002 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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