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Disease Markers
Volume 25 (2008), Issue 4-5, Pages 243-258
http://dx.doi.org/10.1155/2008/515318

Glycomic Approach for Potential Biomarkers on Prostate Cancer: Profiling of N-Linked Glycans in Human Sera and pRNS Cell Lines

Maria Lorna A. de Leoz,1 Hyun Joo An,1 Scott Kronewitter,1 Jaehan Kim,2 Sean Beecroft,1 Ruth Vinall,3 Suzanne Miyamoto,4 Ralph de Vere White,4 Kit S. Lam,4 and Carlito Lebrilla1,5

1Department of Chemistry, University of California Davis, Davis, CA, USA
2Department of Viticulture and Enology, University of California Davis, Davis, CA, USA
3Department of Urology, University of California Davis Medical Center, Sacramento, CA, USA
4Division of Hematology and Oncology, University of California Davis Medical Center, Sacramento, CA, USA
5Department of Biochemistry, School of Medicine, University of California Davis, Davis, CA, USA

Received 24 December 2008; Accepted 24 December 2008

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Prostate cancer is a leading cause of cancer death among men. Currently available screening test measures prostate-specific antigen (PSA) to detect prostate cancer. However, this test produces false positive values that often lead to negative biopsies. Therefore, a more reliable diagnostic tool is needed. Glycans in serum are of particular interest as around half of all proteins are glycosylated. In this study, N-linked glycans were enzymatically released by PNGase F from prostate epithelial cell lines (pRNS) expressing wild type or mutant androgen receptors and a small set of human serum samples. Released glycans were purified and partitioned into neutral and acidic components by solid phase extraction (SPE) using graphitized carbon cartridges. The SPE fractions were analyzed by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI FT-ICR MS). Significant changes in some high-mannose and fucosylated biantennary complex N-linked glycans were observed in the serum of prostate cancer patients.