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Disease Markers
Volume 26, Issue 1, Pages 1-7

IL-1β in Irrigation Fluid and MRNA Expression in Synovial Tissue of the Knee Joint as Therapeutic Markers of Inflammation in Collagen Antibody-Induced Arthritis

Wei-Tso Chia,1,2 Li-Tzu Yeh,2 Yuan-Wu Chen,2 Herng-Sheng Lee,2 Deh-Ming Chang,2 and Huey-Kang Sytwu2

1Hsin Chu General Hospital, Department of Health, Executive Yuan, Taiwan
2Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan

Received 20 February 2009; Accepted 20 February 2009

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective: We studied the relationship between the severity of inflammation and IL-1β production and relative expression level of IL-1β mRNA in irrigation fluid and synovial tissue obtained from the knee joint during the acute stage of a murine model of type II collagen antibody-induced arthritis (CAIA). This model is used to identify potential therapeutic markers for treating rheumatoid arthritis.

Methods: Irrigation fluid and synovium tissue were harvested from the knee joint of BALB/c mice in acute stage of CAIA induction. The IL-1β protein level was measured by enzyme-linked immunosorbent assay, and the relative expression level of IL-1β mRNA was analyzed by reverse transcription-polymerase chain reaction. Two investigators analyzed expression levels and histopathological changes.

Results: IL-1β concentration was higher in irrigation fluid from the knee joint than in the serum in the acute stage of CAIA. The relative expression level of IL-1β mRNA was elevated in synovial tissue. Histopathological changes in the knee joint and foot indicated similar severity.

Conclusions: IL-1β concentration in irrigation fluid and relative expression level of IL-1β mRNA in the synovium have potential as therapeutic markers in studying and treating CAIA.